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51 lines
1.4 KiB
Python
51 lines
1.4 KiB
Python
#!/usr/bin/env python
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"""
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Count total base coverage.
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usage: %prog in_file out_file
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-1, --cols1=N,N,N,N: Columns for start, end, strand in first file
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"""
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from galaxy import eggs
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import pkg_resources
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pkg_resources.require( "bx-python" )
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import sys, traceback, fileinput
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from warnings import warn
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from bx.intervals import *
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from bx.intervals.io import *
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from bx.intervals.operations.base_coverage import *
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from bx.cookbook import doc_optparse
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from galaxy.tools.util.galaxyops import *
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assert sys.version_info[:2] >= ( 2, 4 )
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def main():
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upstream_pad = 0
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downstream_pad = 0
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options, args = doc_optparse.parse( __doc__ )
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try:
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chr_col_1, start_col_1, end_col_1, strand_col_1 = parse_cols_arg( options.cols1 )
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in_fname, out_fname = args
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except:
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doc_optparse.exception()
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g1 = NiceReaderWrapper( fileinput.FileInput( in_fname ),
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chrom_col=chr_col_1,
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start_col=start_col_1,
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end_col=end_col_1,
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strand_col = strand_col_1,
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fix_strand=True )
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try:
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bases = base_coverage(g1)
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except ParseError, exc:
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fail( "Invalid file format: %s" % str( exc ) )
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out_file = open( out_fname, "w" )
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out_file.write( "%s\n" % str( bases ) )
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out_file.close()
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if g1.skipped > 0:
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print skipped( g1, filedesc="" )
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if __name__ == "__main__":
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main()
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