eXpress tool wrapped in Galaxy. Added in "NGS: RNA Analysis".

This commit is contained in:
Remi Marenco
2012-07-17 17:12:51 -04:00
parent a7ef6336ac
commit dd7f9dc3a7
2 changed files with 54 additions and 0 deletions
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<tool file="ngs_rna/tophat_color_wrapper.xml" />
<tool file="ngs_rna/cufflinks_wrapper.xml" />
<tool file="ngs_rna/cuffcompare_wrapper.xml" />
<tool file="ngs_rna/express_wrapper.xml" />
<tool file="ngs_rna/cuffmerge_wrapper.xml" />
<tool file="ngs_rna/cuffdiff_wrapper.xml" />
<!-- Trinity is very memory-intensive and should only be enabled/run
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<tool id="express" name="eXpress" version="1.1.1">
<description>Quantify the abundances of a set of target sequences from sampled subsequences</description>
<requirements>
<requirement type="binary">eXpress</requirement>
</requirements>
<command>express --no-update-check $multiFasta $bamFile</command>
<inputs>
<param format="fasta" name="multiFasta" type="data" label="A set of target references (annotation) in multi-FASTA format" help="The multi-FASTA file can also be a fasta file" />
<param format="bam" name="bamFile" type="data" label="Alignments in the BAM format" help="The set of aligned reads" />
</inputs>
<outputs>
<data format="txt" name="params" from_work_dir="params.xprs"/>
<data format="txt" name="results" from_work_dir="results.xprs"/>
</outputs>
<tests>
<!-- Test for the most simple case : Running eXpress with a .bam file and a .fasta file -->
<test>
<!-- TopHat commands:
eXpress hits.bam Trinity.fasta
-->
<param name="bamFile" ftype="bam" value="eXpress_hits.bam"/>
<param name="multiFasta" ftype="fasta" value="eXpress_Trinity.fasta"/>
<output name="params" file="eXpress_params.xprs" />
<output name="results" file="eXpress_results.xprs"/>
</test>
</tests>
<help>
**eXpress Overview**
eXpress is a streaming tool for quantifying the abundances of a set of target sequences from sampled subsequences. Example applications include transcript-level RNA-Seq quantification, allele-specific/haplotype expression analysis (from RNA-Seq), transcription factor binding quantification in ChIP-Seq, and analysis of metagenomic data.
.. _Ensembl: http://bio.math.berkeley.edu/eXpress/
-----
**Input format**
eXpress requires two input files:
- A multi-FASTA file containing the transcript sequences.
- Read alignments to the multi-FASTA file in BAM format.
------
**Outputs**
- The output for eXpress is saved in a file called results.xprs in an easy-to-parse tab-delimited format.
- Also, params.xprs contains the values of the other parameters (besides abundances and counts) estimated by eXpress.
</help>
</tool>