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eXpress tool wrapped in Galaxy. Added in "NGS: RNA Analysis".
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@@ -361,6 +361,7 @@
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<tool file="ngs_rna/tophat_color_wrapper.xml" />
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<tool file="ngs_rna/cufflinks_wrapper.xml" />
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<tool file="ngs_rna/cuffcompare_wrapper.xml" />
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<tool file="ngs_rna/express_wrapper.xml" />
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<tool file="ngs_rna/cuffmerge_wrapper.xml" />
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<tool file="ngs_rna/cuffdiff_wrapper.xml" />
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<!-- Trinity is very memory-intensive and should only be enabled/run
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@@ -0,0 +1,53 @@
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<tool id="express" name="eXpress" version="1.1.1">
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<description>Quantify the abundances of a set of target sequences from sampled subsequences</description>
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<requirements>
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<requirement type="binary">eXpress</requirement>
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</requirements>
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<command>express --no-update-check $multiFasta $bamFile</command>
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<inputs>
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<param format="fasta" name="multiFasta" type="data" label="A set of target references (annotation) in multi-FASTA format" help="The multi-FASTA file can also be a fasta file" />
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<param format="bam" name="bamFile" type="data" label="Alignments in the BAM format" help="The set of aligned reads" />
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</inputs>
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<outputs>
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<data format="txt" name="params" from_work_dir="params.xprs"/>
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<data format="txt" name="results" from_work_dir="results.xprs"/>
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</outputs>
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<tests>
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<!-- Test for the most simple case : Running eXpress with a .bam file and a .fasta file -->
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<test>
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<!-- TopHat commands:
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eXpress hits.bam Trinity.fasta
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-->
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<param name="bamFile" ftype="bam" value="eXpress_hits.bam"/>
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<param name="multiFasta" ftype="fasta" value="eXpress_Trinity.fasta"/>
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<output name="params" file="eXpress_params.xprs" />
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<output name="results" file="eXpress_results.xprs"/>
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</test>
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</tests>
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<help>
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**eXpress Overview**
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eXpress is a streaming tool for quantifying the abundances of a set of target sequences from sampled subsequences. Example applications include transcript-level RNA-Seq quantification, allele-specific/haplotype expression analysis (from RNA-Seq), transcription factor binding quantification in ChIP-Seq, and analysis of metagenomic data.
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.. _Ensembl: http://bio.math.berkeley.edu/eXpress/
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-----
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**Input format**
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eXpress requires two input files:
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- A multi-FASTA file containing the transcript sequences.
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- Read alignments to the multi-FASTA file in BAM format.
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------
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**Outputs**
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- The output for eXpress is saved in a file called results.xprs in an easy-to-parse tab-delimited format.
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- Also, params.xprs contains the values of the other parameters (besides abundances and counts) estimated by eXpress.
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</help>
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</tool>
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