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update xml file.
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@@ -78,39 +78,58 @@ Accept the following two file formats:
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- fasta: for **454** and **SOLiD** data
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- tabular: for **Solexa** data. Only take the maximal value from each 4 quality scores.
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If *minimal trimmed length to report* is set to a number larger than zero, the tool ouputs any substrings that are longer than this threshold.
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If *minimal length of trimmed reads to report* is set to a number larger than zero, the tool ouputs any substrings that are longer than this threshold.
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If there are more than one substring, the tool outputs all of them in separated fasta format.
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-----
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**Example 1.**
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454 and SOLiD data::
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For 454 and SOLiD files
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- the sequence file is fasta format as following::
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>seq1
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CCGGTATCCG
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>seq1
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- and the score file is also a fasta-like format::
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>quality score for seq1
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33 19 34 25 28 28 28 32 15 34
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454 and SOLiD Output (trimmed by quality score 20 and returned the longest substring)::
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- if use **20** as *minimal quality score*, and
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- use **0** as *minimal length of trimmed reads to report*
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- the output will return the longest sub-sequence::
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>seq1
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GGTATC
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Solexa data::
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-----
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**Example 2.**
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For Solexa files
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- the sequence file is fasta format as following::
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5 300 902 419 TTCTTACCTATTAGTGGTTGAACATC
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- and the score file is also a fasta-like format::
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Solexa quality score (only showed the maximum value)::
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40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 15 40
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Solexa Output (trimmed by quality score 20 and returned the longest substring)::
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Note: only showed the maximum score here
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- if use **20** as *minimal quality score*, and
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- use **0** as *minimal length of trimmed reads to report*
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- the output will return the longest sub-sequence::
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>15774
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TTCTTACCTATTAGTGGTTGAACA
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The fasta title will be replaced by a unique number in Solexa output.
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Note: The fasta title will be replaced by a unique number in Solexa output.
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</help>
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</tool>
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