Files
galaxy/scripts/microbes/create_nib_seq_loc_file.py
T

90 lines
2.8 KiB
Python

#!/usr/bin/env python
# Dan Blankenberg
from __future__ import print_function
import os
import sys
assert sys.version_info[:2] >= (2, 6)
def __main__():
base_dir = os.path.join(os.getcwd(), "bacteria")
try:
base_dir = sys.argv[1]
except IndexError:
print("using default base_dir:", base_dir)
loc_out = os.path.join(base_dir, "seq.loc")
try:
loc_out = os.path.join(base_dir, sys.argv[2])
except Exception:
print("using default seq.loc:", loc_out)
organisms = {}
loc_out = open(loc_out, 'wb')
for result in os.walk(base_dir):
this_base_dir, sub_dirs, files = result
for file in files:
if file[-5:] == ".info":
tmp_dict = {}
info_file = open(os.path.join(this_base_dir, file), 'r')
info = info_file.readlines()
info_file.close()
for line in info:
fields = line.replace("\n", "").split("=")
tmp_dict[fields[0]] = "=".join(fields[1:])
if 'genome project id' in tmp_dict.keys():
name = tmp_dict['genome project id']
if 'build' in tmp_dict.keys():
name = tmp_dict['build']
if name not in organisms.keys():
organisms[name] = {'chrs': {}, 'base_dir': this_base_dir}
for key in tmp_dict.keys():
organisms[name][key] = tmp_dict[key]
else:
if tmp_dict['organism'] not in organisms.keys():
organisms[tmp_dict['organism']] = {'chrs': {}, 'base_dir': this_base_dir}
organisms[tmp_dict['organism']]['chrs'][tmp_dict['chromosome']] = tmp_dict
for org in organisms:
org = organisms[org]
try:
build = org['genome project id']
except KeyError:
continue
if 'build' in org:
build = org['build']
seq_path = os.path.join(org['base_dir'], "seq")
# create seq dir, if exists go to next org
# TODO: add better checking, i.e. for updating
try:
os.mkdir(seq_path)
except Exception:
print("Skipping", build)
# continue
loc_out.write("seq %s %s\n" % (build, seq_path))
# Print org info
for chr in org['chrs']:
chr = org['chrs'][chr]
fasta_file = os.path.join(org['base_dir'], "%s.fna" % chr['chromosome'])
nib_out_file = os.path.join(seq_path, "%s.nib " % chr['chromosome'])
# create nibs using faToNib binary
# TODO: when bx supports writing nib, use it here instead
command = "faToNib %s %s" % (fasta_file, nib_out_file)
os.system(command)
loc_out.close()
if __name__ == "__main__":
__main__()