Files
galaxy/tools/multivariate_stats/cca.py
T

159 lines
5.0 KiB
Python

#!/usr/bin/env python
from galaxy import eggs
import sys, string
from rpy import *
import numpy
def stop_err(msg):
sys.stderr.write(msg)
sys.exit()
infile = sys.argv[1]
x_cols = sys.argv[2].split(',')
y_cols = sys.argv[3].split(',')
x_scale = x_center = "FALSE"
if sys.argv[4] == 'both':
x_scale = x_center = "TRUE"
elif sys.argv[4] == 'center':
x_center = "TRUE"
elif sys.argv[4] == 'scale':
x_scale = "TRUE"
y_scale = y_center = "FALSE"
if sys.argv[5] == 'both':
y_scale = y_center = "TRUE"
elif sys.argv[5] == 'center':
y_center = "TRUE"
elif sys.argv[5] == 'scale':
y_scale = "TRUE"
std_scores = "FALSE"
if sys.argv[6] == "yes":
std_scores = "TRUE"
outfile = sys.argv[7]
outfile2 = sys.argv[8]
fout = open(outfile,'w')
elems = []
for i, line in enumerate( file ( infile )):
line = line.rstrip('\r\n')
if len( line )>0 and not line.startswith( '#' ):
elems = line.split( '\t' )
break
if i == 30:
break # Hopefully we'll never get here...
if len( elems )<1:
stop_err( "The data in your input dataset is either missing or not formatted properly." )
x_vals = []
for k,col in enumerate(x_cols):
x_cols[k] = int(col)-1
x_vals.append([])
y_vals = []
for k,col in enumerate(y_cols):
y_cols[k] = int(col)-1
y_vals.append([])
skipped = 0
for ind,line in enumerate( file( infile )):
if line and not line.startswith( '#' ):
try:
fields = line.strip().split("\t")
valid_line = True
for col in x_cols+y_cols:
try:
assert float(fields[col])
except:
skipped += 1
valid_line = False
break
if valid_line:
for k,col in enumerate(x_cols):
try:
xval = float(fields[col])
except:
xval = NaN#
x_vals[k].append(xval)
for k,col in enumerate(y_cols):
try:
yval = float(fields[col])
except:
yval = NaN#
y_vals[k].append(yval)
except:
skipped += 1
x_vals1 = numpy.asarray(x_vals).transpose()
y_vals1 = numpy.asarray(y_vals).transpose()
x_dat= r.list(array(x_vals1))
y_dat= r.list(array(y_vals1))
try:
r.suppressWarnings(r.library("yacca"))
except:
stop_err("Missing R library yacca.")
set_default_mode(NO_CONVERSION)
try:
xcolnames = ["c%d" %(el+1) for el in x_cols]
ycolnames = ["c%d" %(el+1) for el in y_cols]
cc = r.cca(x=x_dat, y=y_dat, xlab=xcolnames, ylab=ycolnames, xcenter=r(x_center), ycenter=r(y_center), xscale=r(x_scale), yscale=r(y_scale), standardize_scores=r(std_scores))
ftest = r.F_test_cca(cc)
except RException, rex:
stop_err("Encountered error while performing CCA on the input data: %s" %(rex))
set_default_mode(BASIC_CONVERSION)
summary = r.summary(cc)
ncomps = len(summary['corr'])
comps = summary['corr'].keys()
corr = summary['corr'].values()
xlab = summary['xlab']
ylab = summary['ylab']
for i in range(ncomps):
corr[comps.index('CV %s' %(i+1))] = summary['corr'].values()[i]
ftest=ftest.as_py()
print >>fout, "#Component\t%s" %("\t".join(["%s" % el for el in range(1,ncomps+1)]))
print >>fout, "#Correlation\t%s" %("\t".join(["%.4g" % el for el in corr]))
print >>fout, "#F-statistic\t%s" %("\t".join(["%.4g" % el for el in ftest['statistic']]))
print >>fout, "#p-value\t%s" %("\t".join(["%.4g" % el for el in ftest['p.value']]))
print >>fout, "#X-Coefficients\t%s" %("\t".join(["%s" % el for el in range(1,ncomps+1)]))
for i,val in enumerate(summary['xcoef']):
print >>fout, "%s\t%s" %(xlab[i], "\t".join(["%.4g" % el for el in val]))
print >>fout, "#Y-Coefficients\t%s" %("\t".join(["%s" % el for el in range(1,ncomps+1)]))
for i,val in enumerate(summary['ycoef']):
print >>fout, "%s\t%s" %(ylab[i], "\t".join(["%.4g" % el for el in val]))
print >>fout, "#X-Loadings\t%s" %("\t".join(["%s" % el for el in range(1,ncomps+1)]))
for i,val in enumerate(summary['xstructcorr']):
print >>fout, "%s\t%s" %(xlab[i], "\t".join(["%.4g" % el for el in val]))
print >>fout, "#Y-Loadings\t%s" %("\t".join(["%s" % el for el in range(1,ncomps+1)]))
for i,val in enumerate(summary['ystructcorr']):
print >>fout, "%s\t%s" %(ylab[i], "\t".join(["%.4g" % el for el in val]))
print >>fout, "#X-CrossLoadings\t%s" %("\t".join(["%s" % el for el in range(1,ncomps+1)]))
for i,val in enumerate(summary['xcrosscorr']):
print >>fout, "%s\t%s" %(xlab[i], "\t".join(["%.4g" % el for el in val]))
print >>fout, "#Y-CrossLoadings\t%s" %("\t".join(["%s" % el for el in range(1,ncomps+1)]))
for i,val in enumerate(summary['ycrosscorr']):
print >>fout, "%s\t%s" %(ylab[i], "\t".join(["%.4g" % el for el in val]))
r.pdf( outfile2, 8, 8 )
#r.plot(cc)
for i in range(ncomps):
r.helio_plot(cc, cv = i+1, main = r.paste("Explained Variance for CV",i+1), type = "variance")
r.dev_off()