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galaxy/tools/regVariation/substitution_rates.xml
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XML

<tool id="subRate1" name="Estimate substitution rates " version="1.0.0">
<description> for non-coding regions</description>
<command interpreter="python">
substitution_rates.py
$input
$out_file1
#if $region.type == "win":
${region.input2} ${region.input2.dbkey} ${region.input2.metadata.chromCol},$region.input2.metadata.startCol,$region.input2.metadata.endCol,$region.input2.metadata.strandCol
#else:
"None"
#end if
</command>
<inputs>
<param format="maf" name="input" type="data" label="Select pair-wise alignment data"/>
<conditional name="region">
<param name="type" type="select" label="Estimate rates corresponding to" multiple="false">
<option value="align">Alignment block</option>
<option value="win">Intervals in your history</option>
</param>
<when value="win">
<param format="interval" name="input2" type="data" label="Choose intervals">
<validator type="unspecified_build" />
</param>
</when>
<when value="align" />
</conditional>
</inputs>
<outputs>
<data format="tabular" name="out_file1" metadata_source="input"/>
</outputs>
<tests>
<test>
<param name="input" value="Interval2Maf_pairwise_out.maf"/>
<param name="type" value="align"/>
<output name="out_file1" file="subRates1.out"/>
</test>
</tests>
<help>
.. class:: infomark
**What it does**
This tool takes a pairwise MAF file as input and estimates substitution rate according to Jukes-Cantor JC69 model. The 3 new columns appended to the output are explained below:
- L: number of nucleotides compared
- N: number of different nucleotides
- p = N/L
-----
.. class:: warningmark
**Note**
Any block/s not containing exactly two sequences, will be omitted.
</help>
</tool>