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188 lines
7.9 KiB
HTML
188 lines
7.9 KiB
HTML
<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN"
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"http://www.w3.org/TR/html4/loose.dtd">
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<html>
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<head>
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<title>Installing Gmaj</title>
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<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1">
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<meta http-equiv="Content-Style-Type" content="text/css">
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<link rel="stylesheet" type="text/css" href="gmaj.css">
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</head>
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<body>
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<p class=vvlarge>
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<h2>Installing Gmaj</h2>
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<p class=vvlarge>
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TABLE OF CONTENTS
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<p class=small>
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<ul class=notop>
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<li><a href="#intro">Introduction</a>
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<li><a href="#install">Installation</a>
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<li><a href="#data">Data Files</a>
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<li><a href="#page">Web Page</a>
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</ul>
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<p class=vlarge>
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<h3><a name="intro">Introduction</a></h3>
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<p>
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Gmaj can be run in two different modes: as a stand-alone
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application (for viewing local data files yourself) or as an
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applet over the world-wide web (to display your data on a server
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for viewing by others). Both forms of the program are
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distributed together, so the initial download and unpacking
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instructions are the same. Setting it up as an applet, however,
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requires some additional steps: ensuring that Gmaj and the data
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files you want to display are accessible to your web server, and
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building a web page to run the applet.
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<p>
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<h3><a name="install">Installation</a></h3>
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<p>
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Gmaj is available for download as a compressed zip archive,
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<code><a href="http://globin.bx.psu.edu/dist/gmaj/gmaj.zip"
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>gmaj.zip</a></code>. This was created with the Java jar tool,
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but the format is compatible with PKUnzip and many other unzip
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programs. Unzipping the archive will produce
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<code>gmaj.jar</code> (a jar file containing the program itself)
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and a <code>docs</code> subdirectory containing some
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documentation files in HTML format. If your unzipper program
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does not preserve the directory structure and complete file
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names from the archive, you may need to move and/or rename the
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documentation files manually in order for the "Help - Manual"
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function to work. Note that the <code>gmaj.jar</code> file does
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not need a second round of unzipping -- Java will access it
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"as is". If you are setting up Gmaj as an applet, be sure to
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unzip the archive in a directory/folder that will be accessible
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to your web server, e.g., a new <code>gmaj</code> directory
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somewhere in the server's document space.
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<p>
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If you are running Gmaj in stand-alone mode, you will also need
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to have Java installed on your computer. For applets, the
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server does not need Java but the end user does; you may want to
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mention this on your Gmaj web page. In both cases <b>Java 1.3
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or higher</b> is required, and for best compatibility
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<a href="http://java.com/en/download/manual.jsp">Sun's JRE</a>
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(or JDK) is recommended.
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<p>
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For stand-alone mode, that's all there is to the installation;
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you will specify different start-up parameters for Gmaj each time
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you run it (see <a href="gmaj_help.html"
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>Starting and Running Gmaj</a>). The remaining sections on this
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page apply only to applet setup.
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<p>
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<h3><a name="data">Data Files</a></h3>
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<p>
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In addition to the alignment data, Gmaj can display several
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kinds of annotations, including genes/exons, repeats, linkbars,
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color underlays, text highlights, and reconstruction scores for
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ancestral sequences, with a meta-data parameters file to tie
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them all together. For detailed descriptions of these files and
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their format requirements, please see <a href="gmaj_input.html"
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>Input Files for Gmaj</a>.
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<p>
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When setting up Gmaj as an applet, the data files must be
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accessible to your web server. Also, due to Java security
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restrictions, they must all be located on the same server as the
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<code>gmaj.jar</code> file, because an applet is normally only
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allowed to contact the same server it was loaded from. We find
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it convenient to group the files for each invocation (e.g., each
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genomic region) in a separate subdirectory of the
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<code>gmaj</code> directory. It is also possible to bundle them
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into a single zipped data file for each invocation, which eases
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both storage requirements and download time (discussed further
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in <a href="gmaj_input.html">Input Files for Gmaj</a>).
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<p>
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<h3><a name="page">Web Page</a></h3>
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<p>
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The last step in setting up the applet is to create a web page on
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your server that invokes it with the appropriate parameters for
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loading your data files. The applet normally appears as a labeled
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button that opens a Gmaj window when the user clicks on it; thus
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you can have several buttons on the same page, each set up to
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display a different set of data. The basic format of the HTML
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code looks like this:
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<blockquote>
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<pre>
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<applet code="edu.psu.bx.gmaj.MajApplet.class"
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archive="gmaj.jar"
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width="200" height="30">
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<param name=paramfile value="/java/gmaj/alpha/demo.gmaj">
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<param name=bundle value="/java/gmaj/alpha/demo.zip">
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<param name=buttonlabel value="Alpha-globin">
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<param name=nobutton value="false">
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<param name=initzoom value="mouse 110000 147000">
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<param name=posturl value="/cgi-bin/save-posted-file.pl">
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<param name=urlpause value="100">
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<param name=debug value="false">
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<i>Your browser is not responding to the &lt;applet&gt; tag.</i>
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</applet>
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</pre>
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</blockquote>
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This particular fragment is based on the alpha-globin example
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from our server; naturally you need to replace the values with
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your own file URLs, button label, etc. A few things to note:
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<ul>
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<li> If the <code>gmaj.jar</code> file is not in the same
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directory as your web page, you'll need to supply the path
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to it in the <code>archive</code> attribute.
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<li> The <code>width</code> and <code>height</code> attributes
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are for the button, not the Gmaj windows.
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<li> You can specify either or both of the first two
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<code><param name=...</code> lines
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(<code>paramfile</code> and <code>bundle</code>); the
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others are optional.
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<li> If the <code>nobutton</code> parameter is set to
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<code>"true"</code>, Gmaj will proceed to open its window
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immediately instead of displaying a start button.
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<li> The <code>initzoom</code> parameter specifies an initial
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zoom setting to be applied when the window opens. The user
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can still invoke the Unzoom or Set Zoom features
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interactively to see the entire sequence range. The
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sequence name must match one of the names from the alignment
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file(s), and the endpoints must include the offset (if any)
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for that sequence from the parameters file. To specify the
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reference sequence without a zoom region, use <code>-1</code>
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for both endpoints.
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<li> The <code>posturl</code> parameter designates a URL on your
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server where exported alignments should be sent. By default
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the Export feature is not available in applet mode, because
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applets generally can't write to the user's local disk due
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to security restrictions. However, by specifying this
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parameter you can enable the applet to send the exported data
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to your server instead (typically a CGI script). The output
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is sent via an HTTP POST request using the MIME protocol for
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web forms; currently for applets the export file format is
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always MAF, and the filename is always
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<code>Gmaj_output.maf</code>.
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<pre class=smallfont>
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Content-Type: multipart/form-data; boundary=______AaB03x
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--______AaB03x
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Content-Disposition: form-data; name=file_data; filename=Gmaj_output.maf
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Content-Type: application/octet-stream
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[MAF file contents, in plain ASCII with platform-dependent line breaks]
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--______AaB03x--
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</pre>
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<li> The <code>urlpause</code> parameter specifies how many
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milliseconds the program should pause before retrieving each
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file from a URL, in order to avoid overloading your server.
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<li> If the <code>debug</code> parameter is set to
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<code>"true"</code>, Gmaj will print a few extra warning
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messages in the browser's Java console if certain problems
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occur. Normally you won't need this, as it is mainly for
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development purposes.
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<li> To create several buttons, just repeat this entire block of
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code (with new parameter values, of course).
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</ul>
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<p>
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<p class=vvlarge>
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<hr>
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<i>Cathy Riemer, June 2008</i>
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</body>
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</html>
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