Files
galaxy/scripts/microbes/create_nib_seq_loc_file.py
T
2022-02-03 07:42:20 -05:00

89 lines
2.8 KiB
Python

#!/usr/bin/env python
# Dan Blankenberg
import os
import sys
assert sys.version_info[:2] >= (2, 6)
def __main__():
base_dir = os.path.join(os.getcwd(), "bacteria")
try:
base_dir = sys.argv[1]
except IndexError:
print("using default base_dir:", base_dir)
loc_out = os.path.join(base_dir, "seq.loc")
try:
loc_out = os.path.join(base_dir, sys.argv[2])
except Exception:
print("using default seq.loc:", loc_out)
organisms = {}
loc_out = open(loc_out, "wb")
for result in os.walk(base_dir):
this_base_dir, sub_dirs, files = result
for file in files:
if file[-5:] == ".info":
tmp_dict = {}
info_file = open(os.path.join(this_base_dir, file))
info = info_file.readlines()
info_file.close()
for line in info:
fields = line.replace("\n", "").split("=")
tmp_dict[fields[0]] = "=".join(fields[1:])
if "genome project id" in tmp_dict.keys():
name = tmp_dict["genome project id"]
if "build" in tmp_dict.keys():
name = tmp_dict["build"]
if name not in organisms.keys():
organisms[name] = {"chrs": {}, "base_dir": this_base_dir}
for key in tmp_dict.keys():
organisms[name][key] = tmp_dict[key]
else:
if tmp_dict["organism"] not in organisms.keys():
organisms[tmp_dict["organism"]] = {"chrs": {}, "base_dir": this_base_dir}
organisms[tmp_dict["organism"]]["chrs"][tmp_dict["chromosome"]] = tmp_dict
for org in organisms:
org = organisms[org]
try:
build = org["genome project id"]
except KeyError:
continue
if "build" in org:
build = org["build"]
seq_path = os.path.join(org["base_dir"], "seq")
# create seq dir, if exists go to next org
# TODO: add better checking, i.e. for updating
try:
os.mkdir(seq_path)
except Exception:
print("Skipping", build)
# continue
loc_out.write(f"seq {build} {seq_path}\n")
# Print org info
for chr in org["chrs"]:
chr = org["chrs"][chr]
fasta_file = os.path.join(org["base_dir"], "%s.fna" % chr["chromosome"])
nib_out_file = os.path.join(seq_path, "%s.nib " % chr["chromosome"])
# create nibs using faToNib binary
# TODO: when bx supports writing nib, use it here instead
command = f"faToNib {fasta_file} {nib_out_file}"
os.system(command)
loc_out.close()
if __name__ == "__main__":
__main__()