diff --git a/config/galaxy.ini.sample b/config/galaxy.ini.sample
index 09f57e758b9..f8b80f362ef 100644
--- a/config/galaxy.ini.sample
+++ b/config/galaxy.ini.sample
@@ -187,6 +187,10 @@ paste.app_factory = galaxy.web.buildapp:app_factory
 # all Conda resolvers, but multiple resolvers can be configured independently
 # in dependency_resolvers_config_file and these options overridden.
 # Location on the filesystem where Conda packages are installed
+
+# conda_prefix is the location on the filesystem where Conda packages and environments are installed
+# IMPORTANT: Due to a current limitation in conda, the total length of the
+# conda_prefix and the job_working_directory path should be less than 50 characters!
 #conda_prefix = <tool_dependency_dir>/_conda
 # Override the Conda executable to use, it will default to the one on the
 # PATH (if available) and then to <conda_prefix>/bin/conda