diff --git a/lib/galaxy/tools/parameters/basic.py b/lib/galaxy/tools/parameters/basic.py
index 2c31daee649..874fd2c2ef7 100644
--- a/lib/galaxy/tools/parameters/basic.py
+++ b/lib/galaxy/tools/parameters/basic.py
@@ -604,6 +604,9 @@ class FileToolParameter(ToolParameter):
                 if re.match(r'^[\w-]+$', session_id) is None:
                     raise ValueError("Invalid session id format.")
                 local_filename = os.path.abspath(os.path.join(upload_store, session_id))
+                if upload_store != trans.app.config.new_file_path and not os.path.exists(local_filename):
+                    # Fallback for old chunked API, remove in 22.05
+                    local_filename = os.path.abspath(os.path.join(trans.app.config.new_file_path, session_id))
             else:
                 # handle nginx upload
                 upload_store = trans.app.config.nginx_upload_store