diff --git a/lib/galaxy/config.py b/lib/galaxy/config.py
index 3924d46ff6c..8919b1b721d 100644
--- a/lib/galaxy/config.py
+++ b/lib/galaxy/config.py
@@ -525,6 +525,7 @@ class Configuration( object ):
             datatypes_config_file=[ 'config/datatypes_conf.xml', 'datatypes_conf.xml', 'config/datatypes_conf.xml.sample' ],
             external_service_type_config_file=[ 'config/external_service_types_conf.xml', 'external_service_types_conf.xml', 'config/external_service_types_conf.xml.sample' ],
             job_config_file=[ 'config/job_conf.xml', 'job_conf.xml' ],
+            tool_destinations_config_file=[ 'config/tool_destinations.yml', 'config/tool_destinations.yml.sample' ],
             job_metrics_config_file=[ 'config/job_metrics_conf.xml', 'job_metrics_conf.xml', 'config/job_metrics_conf.xml.sample' ],
             dependency_resolvers_config_file=[ 'config/dependency_resolvers_conf.xml', 'dependency_resolvers_conf.xml' ],
             job_resource_params_file=[ 'config/job_resource_params_conf.xml', 'job_resource_params_conf.xml' ],
diff --git a/lib/galaxy/jobs/rules/dynamic_tool_destination.py b/lib/galaxy/jobs/rules/dynamic_tool_destination.py
index 3116b6be9ee..408c01a422c 100755
--- a/lib/galaxy/jobs/rules/dynamic_tool_destination.py
+++ b/lib/galaxy/jobs/rules/dynamic_tool_destination.py
@@ -1103,7 +1103,7 @@ def importer(test):
 
 
 def map_tool_to_destination(
-        job, app, tool, user_email, test=False, path="/config/tool_destinations.yml"):
+        job, app, tool, user_email, test=False, path=None):
     """
     Dynamically allocate resources
 
@@ -1128,6 +1128,9 @@ def map_tool_to_destination(
     records_rule_present = False
 
     # Get configuration from tool_destinations.yml
+    if path is None:
+        path = app.config.tool_destinations_config_file
+
     try:
         config = parse_yaml(path)
     except MalformedYMLException as e: