diff --git a/lib/galaxy/datatypes/sequence.py b/lib/galaxy/datatypes/sequence.py
index ffa11e25a2f..28fbe4754be 100644
--- a/lib/galaxy/datatypes/sequence.py
+++ b/lib/galaxy/datatypes/sequence.py
@@ -694,13 +694,16 @@ class BaseFastq(Sequence):
         >>> fname = get_test_fname('1.fastqsanger')
         >>> FastqSanger().sniff(fname)
         True
+        >>> fname = get_test_fname('4.fastqsanger')
+        >>> FastqSanger().sniff(fname)
+        True
         >>> fname = get_test_fname('3.fastq')
         >>> FastqSanger().sniff(fname)
         False
         >>> Fastq().sniff(fname)
         True
         >>> fname = get_test_fname('2.fastq')
-        >>> Fastq().sniff( fname )
+        >>> Fastq().sniff(fname)
         True
         >>> FastqSanger().sniff(fname)
         False
@@ -819,7 +822,7 @@ class FastqSanger(Fastq):
     def quality_check(lines):
         """Presuming lines are lines from a fastq file, return True if the qualities are compatible with sanger encoding"""
         for line in islice(lines, 3, None, 4):
-            if not all(_ >= '!' and _ <= 'M' for _ in line[0]) or ' ' in line:
+            if not all(_ >= '!' and _ <= 'S' for _ in line[0]):
                 return False
         return True
 
diff --git a/lib/galaxy/datatypes/test/4.fastqsanger b/lib/galaxy/datatypes/test/4.fastqsanger
new file mode 100644
index 00000000000..ff60d92d068
--- /dev/null
+++ b/lib/galaxy/datatypes/test/4.fastqsanger
@@ -0,0 +1,8 @@
+@DCW97JN1:309:C0C42ACXX:4:2206:12976:57510/1
+AAATGGGCATAATATAGATGTAGAGATGTGTTGAATTTATGACTCATTT
++
+ADECJJJJDCCECCCCMCCNCCMCNBCLBJBCLADCDC=CNDJILEDDD
+@DCW97JN1:309:C0C42ACXX:4:2211:6915:3569/1
+AAAGAAATTAAATGGGCATAATATAGATGTAGAGATGTGTTGAATTTAT
++
+@DEJAAA?B@ECCEFFGDBBFC8ABGCBK=BI=66@HE@@JACCCDCCI