diff --git a/lib/galaxy/tool_util/verify/__init__.py b/lib/galaxy/tool_util/verify/__init__.py
index cac554dd0b7..66dda2ceda4 100644
--- a/lib/galaxy/tool_util/verify/__init__.py
+++ b/lib/galaxy/tool_util/verify/__init__.py
@@ -595,6 +595,12 @@ def files_image_diff(file1: str, file2: str, attributes: Optional[Dict[str, Any]
     if arr1.shape != arr2.shape:
         raise AssertionError(f"Image dimensions did not match ({arr1.shape}, {arr2.shape}).")
 
+    # Handle `bool` images by converting them to `uint8`
+    if numpy.issubdtype(arr1.dtype, bool):
+        arr1 = arr1.astype(numpy.uint8)
+    if numpy.issubdtype(arr2.dtype, bool):
+        arr2 = arr2.astype(numpy.uint8)
+
     distance = get_image_metric(attributes)(arr1, arr2)
     distance_eps = attributes.get("eps", DEFAULT_EPS)
     if distance > distance_eps:
diff --git a/test/functional/tools/image_diff.xml b/test/functional/tools/image_diff.xml
index addb4ef24b4..d6d9f9b1ffb 100644
--- a/test/functional/tools/image_diff.xml
+++ b/test/functional/tools/image_diff.xml
@@ -27,6 +27,11 @@
       <param name="in" value="im4_float.tif" />
       <output name="out" value="im4_float.tif" compare="image_diff" />
     </test>
+    <!-- test pair of equal images (bool tiff) -->
+    <test>
+      <param name="in" value="im3_b_bool.tiff" />
+      <output name="out" value="im3_b_bool.tiff" compare="image_diff" metric="iou" pin_labels="0" />
+    </test>
     <!-- test pair of different images -->
     <test>
       <param name="in" value="im2_a.png" />