diff --git a/lib/galaxy/tools/parameters/basic.py b/lib/galaxy/tools/parameters/basic.py
index 83ee4ea2135..ad3fc28c9a6 100644
--- a/lib/galaxy/tools/parameters/basic.py
+++ b/lib/galaxy/tools/parameters/basic.py
@@ -651,9 +651,6 @@ class FileToolParameter(ToolParameter):
                 if re.match(r"^[\w-]+$", session_id) is None:
                     raise ValueError("Invalid session id format.")
                 local_filename = os.path.abspath(os.path.join(upload_store, session_id))
-                if upload_store != trans.app.config.new_file_path and not os.path.exists(local_filename):
-                    # Fallback for old chunked API, remove in 22.05
-                    local_filename = os.path.abspath(os.path.join(trans.app.config.new_file_path, session_id))
             else:
                 # handle nginx upload
                 upload_store = trans.app.config.nginx_upload_store
diff --git a/lib/galaxy/webapps/galaxy/api/uploads.py b/lib/galaxy/webapps/galaxy/api/uploads.py
index 0ed40d90ee2..dff42c5269d 100644
--- a/lib/galaxy/webapps/galaxy/api/uploads.py
+++ b/lib/galaxy/webapps/galaxy/api/uploads.py
@@ -2,14 +2,8 @@
 API operations for uploaded files in storage.
 """
 import logging
-import os
-import re
 
-from galaxy import exceptions
-from galaxy.web.framework.decorators import (
-    expose_api_raw_anonymous,
-    legacy_expose_api_anonymous,
-)
+from galaxy.web.framework.decorators import expose_api_raw_anonymous
 from . import BaseGalaxyAPIController
 
 log = logging.getLogger(__name__)
@@ -25,39 +19,3 @@ class UploadsAPIController(BaseGalaxyAPIController):
         """
         # Internal endpoint, only purpose is to authenticate user, but may grow additional functionality in the future
         return None
-
-    @legacy_expose_api_anonymous
-    def index(self, trans, **kwd):
-        raise exceptions.NotImplemented("Listing uploads is not implemented.")
-
-    @legacy_expose_api_anonymous
-    def create(self, trans, payload, **kwd):
-        """
-        POST /api/uploads/
-        """
-        session_id = payload.get("session_id")
-        session_start = payload.get("session_start")
-        session_chunk = payload.get("session_chunk")
-        if re.match(r"^[\w-]+$", session_id) is None:
-            raise exceptions.MessageException("Requires a session id.")
-        if session_start is None:
-            raise exceptions.MessageException("Requires a session start.")
-        if not hasattr(session_chunk, "file"):
-            raise exceptions.MessageException("Requires a session chunk.")
-        target_file = os.path.join(trans.app.config.new_file_path, session_id)
-        target_size = 0
-        if os.path.exists(target_file):
-            target_size = os.path.getsize(target_file)
-        if session_start != target_size:
-            raise exceptions.MessageException("Incorrect session start.")
-        chunk_size = os.fstat(session_chunk.file.fileno()).st_size
-        if chunk_size > trans.app.config.chunk_upload_size:
-            raise exceptions.MessageException("Invalid chunk size.")
-        with open(target_file, "ab") as f:
-            while True:
-                read_chunk = session_chunk.file.read(self.READ_CHUNK_SIZE)
-                if not read_chunk:
-                    break
-                f.write(read_chunk)
-        session_chunk.file.close()
-        return {"message": "Successful."}