diff --git a/lib/galaxy/config/sample/datatypes_conf.xml.sample b/lib/galaxy/config/sample/datatypes_conf.xml.sample
index cfd58ef2285..dd056fdca9e 100644
--- a/lib/galaxy/config/sample/datatypes_conf.xml.sample
+++ b/lib/galaxy/config/sample/datatypes_conf.xml.sample
@@ -185,6 +185,7 @@
     <datatype extension="grib" type="galaxy.datatypes.binary:Grib" mimetype="application/octet-stream" display_in_upload="true"/>
     <datatype extension="loom" type="galaxy.datatypes.binary:Loom" description="An HDF5-based Loom File" mimetype="application/octet-stream" display_in_upload="true"/>
     <datatype extension="h5ad" type="galaxy.datatypes.binary:Anndata" description="An HDF5-based anndata File" mimetype="application/octet-stream" display_in_upload="true"/>
+    <datatype extension="visium.tar.gz" type="galaxy.datatypes.binary:Visium" subclass="true" display_in_upload="true" description="Visium is a tar.gz archive with at least a 'Spatial' subfolder, a filtered h5 file and a raw h5 file." />
     <datatype extension="mz5" type="galaxy.datatypes.binary:H5" subclass="true" mimetype="application/octet-stream" display_in_upload="true"/>
     <datatype extension="hyphy_results.json" type="galaxy.datatypes.text:Json" mimetype="application/json" subclass="true" display_in_upload="false"/>
     <datatype extension="hivtrace" type="galaxy.datatypes.text:Json" mimetype="application/json" subclass="true" display_in_upload="false"/>
diff --git a/lib/galaxy/datatypes/binary.py b/lib/galaxy/datatypes/binary.py
index b56c7bee39e..de4565a58c3 100644
--- a/lib/galaxy/datatypes/binary.py
+++ b/lib/galaxy/datatypes/binary.py
@@ -318,6 +318,29 @@ class Meryldb(CompressedArchive):
         return False
 
 
+class Visium(CompressedArchive):
+    """Visium is a tar.gz archive with at least a 'Spatial' subfolder, a filtered h5 file and a raw h5 file."""
+
+    file_ext = "visium.tar.gz"
+
+    def sniff(self, filename: str) -> bool:
+        """
+        Check data structure:
+        Contains h5 files
+        Contains spatial folder
+        """
+        try:
+            if filename and tarfile.is_tarfile(filename):
+                with tarfile.open(filename, "r") as temptar:
+                    _tar_content = temptar.getnames()
+                    if "spatial" in _tar_content:
+                        if len([_ for _ in _tar_content if _.endswith("matrix.h5")]) == 2:
+                            return True
+        except Exception as e:
+            log.warning("%s, sniff Exception: %s", self, e)
+        return False
+
+
 class Bref3(Binary):
     """Bref3 format is a binary format for storing phased, non-missing genotypes for a list of samples."""