diff --git a/lib/galaxy/dependencies/pinned-requirements.txt b/lib/galaxy/dependencies/pinned-requirements.txt
index 4a98d727db5..808cc037714 100644
--- a/lib/galaxy/dependencies/pinned-requirements.txt
+++ b/lib/galaxy/dependencies/pinned-requirements.txt
@@ -67,7 +67,6 @@ importlib-metadata==4.10.1; python_version == "3.7" and (python_version >= "3.7"
 importlib-resources==5.4.0; python_version >= "3.6"
 isa-rwval==0.10.10
 isodate==0.6.1; python_version >= "3.7" and python_version < "4"
-isort==5.10.1; python_full_version >= "3.6.1" and python_version < "4.0"
 jsonschema==4.4.0; python_version >= "3.7"
 kombu==5.2.3; python_version >= "3.7"
 lagom==1.7.0; python_version >= "3.6" and python_version < "4.0"
diff --git a/pyproject.toml b/pyproject.toml
index f15c7bf6c54..fe6c0bd55d1 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -100,7 +100,6 @@ uvloop = "*"
 WebOb = "*"
 Whoosh = "*"
 zipstream-new = "*"
-isort = "^5.10.1"
 
 [tool.poetry.dev-dependencies]
 black = "^22.1.0"
@@ -108,6 +107,7 @@ cwltest = "2.2.20210901154959"
 darker = "*"
 fluent-logger = "*"
 httpx = "*"
+isort = "^5.10.1"
 lxml = "!=4.2.2"
 markdown-it-reporter = "*"
 NoseHTML = "*"