diff --git a/lib/galaxy/config/__init__.py b/lib/galaxy/config/__init__.py
index b3253dcd39e..e68860eb977 100644
--- a/lib/galaxy/config/__init__.py
+++ b/lib/galaxy/config/__init__.py
@@ -740,7 +740,6 @@ class GalaxyAppConfiguration(BaseAppConfiguration, CommonConfigurationMixin):
             for ip in kwargs.get("fetch_url_allowlist", "").split(',')
             if len(ip.strip()) > 0
         ]
-        self.template_path = self._in_root_dir(kwargs.get("template_path", "templates"))
         self.job_queue_cleanup_interval = int(kwargs.get("job_queue_cleanup_interval", "5"))
         self.cluster_files_directory = self._in_root_dir(self.cluster_files_directory)
 
diff --git a/lib/galaxy/webapps/reports/config.py b/lib/galaxy/webapps/reports/config.py
index 5f653fbe35d..1f65b90833b 100644
--- a/lib/galaxy/webapps/reports/config.py
+++ b/lib/galaxy/webapps/reports/config.py
@@ -33,7 +33,6 @@ class Configuration:
         self.id_secret = kwargs.get("id_secret", "USING THE DEFAULT IS NOT SECURE!")
         self.use_remote_user = string_as_bool(kwargs.get("use_remote_user", "False"))
         self.require_login = string_as_bool(kwargs.get("require_login", "False"))
-        self.template_path = resolve_path(kwargs.get("template_path", "templates"), self.root)
         self.template_cache_path = resolve_path(kwargs.get("template_cache_path", "database/compiled_templates/reports"), self.root)
         self.allow_user_creation = string_as_bool(kwargs.get("allow_user_creation", "True"))
         self.allow_user_deletion = string_as_bool(kwargs.get("allow_user_deletion", "False"))
@@ -66,7 +65,7 @@ class Configuration:
 
     def check(self):
         # Check that required directories exist
-        for path in self.root, self.template_path:
+        for path in (self.root, ):
             if not os.path.isdir(path):
                 raise ConfigurationError(f"Directory does not exist: {path}")
 
diff --git a/lib/galaxy_test/driver/driver_util.py b/lib/galaxy_test/driver/driver_util.py
index aff24b1abb7..07e8ad2a81b 100644
--- a/lib/galaxy_test/driver/driver_util.py
+++ b/lib/galaxy_test/driver/driver_util.py
@@ -249,7 +249,6 @@ def setup_galaxy_config(
         master_api_key=master_api_key,
         running_functional_tests=True,
         template_cache_path=template_cache_path,
-        template_path='templates',
         tool_config_file=tool_config_file,
         tool_data_table_config_path=tool_data_table_config_path,
         tool_parse_help=False,
diff --git a/lib/tool_shed/test/functional_tests.py b/lib/tool_shed/test/functional_tests.py
index 2e35eaf3488..5bc08abc79a 100644
--- a/lib/tool_shed/test/functional_tests.py
+++ b/lib/tool_shed/test/functional_tests.py
@@ -89,7 +89,6 @@ class ToolShedTestDriver(driver_util.TestDriver):
                       shed_tool_data_table_config=shed_tool_data_table_conf_file,
                       smtp_server='smtp.dummy.string.tld',
                       email_from='functional@localhost',
-                      template_path='templates',
                       tool_parse_help=False,
                       use_heartbeat=False)
         kwargs.update(toolshed_database_conf)
diff --git a/lib/tool_shed/webapp/config.py b/lib/tool_shed/webapp/config.py
index cdbb987e126..d1ad23d608b 100644
--- a/lib/tool_shed/webapp/config.py
+++ b/lib/tool_shed/webapp/config.py
@@ -20,9 +20,6 @@ from galaxy.web.formatting import expand_pretty_datetime_format
 
 log = logging.getLogger(__name__)
 
-ts_webapp_path = os.path.abspath(os.path.dirname(__file__))
-templates_path = os.path.join(ts_webapp_path, 'templates')
-
 TOOLSHED_APP_NAME = 'tool_shed'
 TOOLSHED_CONFIG_SCHEMA_PATH = 'lib/tool_shed/webapp/config_schema.yml'
 
@@ -48,7 +45,6 @@ class ToolShedAppConfiguration(BaseAppConfiguration, CommonConfigurationMixin):
         paths_to_check = [
             self.file_path,
             self.hgweb_config_dir,
-            self.template_path,
             self.tool_data_path,
             self.template_cache_path,
             os.path.join(self.tool_data_path, 'shared', 'jars'),
@@ -95,7 +91,6 @@ class ToolShedAppConfiguration(BaseAppConfiguration, CommonConfigurationMixin):
         self.registration_warning_message = kwargs.get('registration_warning_message')
         self.email_domain_blocklist_content = None
         self.email_domain_allowlist_content = None
-        self.template_path = templates_path
         self.template_cache_path = self._in_root_dir(kwargs.get('template_cache_path', 'database/compiled_templates/community'))
         self.error_email_to = kwargs.get('error_email_to')
         self.pretty_datetime_format = expand_pretty_datetime_format(self.pretty_datetime_format)
diff --git a/test/unit/webapps/test_routes.py b/test/unit/webapps/test_routes.py
index 20be16f7cea..7fe1485395a 100644
--- a/test/unit/webapps/test_routes.py
+++ b/test/unit/webapps/test_routes.py
@@ -22,7 +22,7 @@ class TestWebapp(WebApplication):
 
 
 def test_galaxy_routes():
-    test_config = Bunch(template_path="/tmp", template_cache_path="/tmp")
+    test_config = Bunch(template_cache_path="/tmp")
     app = Bunch(config=test_config, security=object(), trace_logger=None, name="galaxy")
     test_webapp = TestWebapp(app)