diff --git a/lib/galaxy/dependencies/pinned-requirements.txt b/lib/galaxy/dependencies/pinned-requirements.txt
index b1b103cc865..16b857d312f 100644
--- a/lib/galaxy/dependencies/pinned-requirements.txt
+++ b/lib/galaxy/dependencies/pinned-requirements.txt
@@ -134,7 +134,7 @@ pycryptodome==3.19.1 ; python_version >= "3.8" and python_version < "3.12"
 pydantic-tes==0.1.5 ; python_version >= "3.8" and python_version < "3.12"
 pydantic==2.5.3 ; python_version >= "3.8" and python_version < "3.12"
 pydantic[email]==2.5.3 ; python_version >= "3.8" and python_version < "3.12"
-pydot==1.4.2 ; python_version >= "3.8" and python_version < "3.12"
+pydot==2.0.0 ; python_version >= "3.8" and python_version < "3.12"
 pyeventsystem==0.1.0 ; python_version >= "3.8" and python_version < "3.12"
 pyfaidx==0.7.2.2 ; python_version >= "3.8" and python_version < "3.12"
 pygments==2.17.2 ; python_version >= "3.8" and python_version < "3.12"