diff --git a/lib/galaxy/dependencies/pipfiles/default/pinned-requirements.txt b/lib/galaxy/dependencies/pipfiles/default/pinned-requirements.txt
index e98273ceaad..fec4f6d9601 100644
--- a/lib/galaxy/dependencies/pipfiles/default/pinned-requirements.txt
+++ b/lib/galaxy/dependencies/pipfiles/default/pinned-requirements.txt
@@ -138,7 +138,7 @@ pyopenssl==19.1.0
 pyparsing==2.4.7
 pyperclip==1.8.0
 pyrsistent==0.16.0
-pysam==0.15.4
+pysam==0.16.0.1
 pysftp==0.2.9
 python-cinderclient==4.0.0
 python-dateutil==2.8.1