diff --git a/lib/galaxy/tool_util/linters/datatypes.py b/lib/galaxy/tool_util/linters/datatypes.py
index cd35f0600fc..6d7c72a8c6b 100644
--- a/lib/galaxy/tool_util/linters/datatypes.py
+++ b/lib/galaxy/tool_util/linters/datatypes.py
@@ -1,4 +1,5 @@
 import os.path
+from os import getenv
 from typing import (
     Set,
     TYPE_CHECKING,
@@ -18,7 +19,7 @@ if TYPE_CHECKING:
     from galaxy.tool_util.parser import ToolSource
     from galaxy.util.resources import Traversable
 
-DATATYPES_CONF = resource_path(__name__, "datatypes_conf.xml.sample")
+DATATYPES_CONF = getenv("DATATYPES_CONF", resource_path(__name__, "datatypes_conf.xml.sample"))
 
 
 def _parse_datatypes(datatype_conf_path: Union[str, "Traversable"]) -> Set[str]: