diff --git a/lib/galaxy/datatypes/binary.py b/lib/galaxy/datatypes/binary.py
index 2a478d7f6df..0bd799db6e2 100644
--- a/lib/galaxy/datatypes/binary.py
+++ b/lib/galaxy/datatypes/binary.py
@@ -399,3 +399,5 @@ class TwoBit (Binary):
             return dataset.peek
         except:
             return "Binary TwoBit format nucleotide file (%s)" % (data.nice_size(dataset.get_size()))
+
+Binary.register_sniffable_binary_format("twobit", "twobit", TwoBit)