diff --git a/lib/galaxy/dependencies/__init__.py b/lib/galaxy/dependencies/__init__.py
index 1664d07e6f0..0bb785aa136 100644
--- a/lib/galaxy/dependencies/__init__.py
+++ b/lib/galaxy/dependencies/__init__.py
@@ -298,7 +298,7 @@ class ConditionalDependencies:
         return self.pkce_support
 
     def check_rucio_clients(self):
-        return sys.version_info >= (3, 9)
+        return "rucio" in self.object_stores and sys.version_info >= (3, 9)
 
 
 def optional(config_file=None):
diff --git a/lib/galaxy/dependencies/conditional-requirements.txt b/lib/galaxy/dependencies/conditional-requirements.txt
index e1fa726ff27..2c9efa7eab4 100644
--- a/lib/galaxy/dependencies/conditional-requirements.txt
+++ b/lib/galaxy/dependencies/conditional-requirements.txt
@@ -61,5 +61,5 @@ weasyprint
 # AWS Batch runner
 boto3
 
-#For Rucio storage plugin
-rucio-clients==33.6.0
\ No newline at end of file
+# For Rucio storage plugin
+rucio-clients>=33.6.0
diff --git a/pyproject.toml b/pyproject.toml
index 2fdd4b31429..0ebb92ddf93 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -153,6 +153,7 @@ pytest-playwright = "*"
 pytest-postgresql = "!=3.0.0"  # https://github.com/ClearcodeHQ/pytest-postgresql/issues/426
 pytest-shard = "*"
 responses = "*"
+rucio-clients = {version = ">=33.6.0", python = ">=3.9"}  # https://github.com/galaxyproject/galaxy/pull/17156
 selenium = "*"
 urllib3 = "<2"  # Necessary for poetry for resolve deps for Python 3.8+
 seletools = "*"