diff --git a/pyproject.toml b/pyproject.toml
index 0a19ddcc294..6788afd0fee 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -133,3 +133,14 @@ testfixtures = "*"
 tuspy = "*"
 twill = "*"
 watchdog = "*"
+
+[tool.isort]
+force_alphabetical_sort_within_sections = true
+multi_line_output = 3
+force_grid_wrap = 2
+include_trailing_comma = true
+src_paths = ['lib']
+known_first_party = ['galaxy_test']
+no_lines_before = ['LOCALFOLDER']
+reverse_relative = true
+skip_gitignore = true