diff --git a/lib/galaxy/dependencies/dev-requirements.txt b/lib/galaxy/dependencies/dev-requirements.txt
index 1513ba45325..e809b654c97 100644
--- a/lib/galaxy/dependencies/dev-requirements.txt
+++ b/lib/galaxy/dependencies/dev-requirements.txt
@@ -1,5 +1,6 @@
 --extra-index-url https://wheels.galaxyproject.org/simple
 
+a2wsgi==1.4.0; python_version >= "3.6" and python_version < "4.0"
 adal==1.2.7
 aiofiles==0.8.0; python_version >= "3.6" and python_version < "4.0"
 alabaster==0.7.12; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6"
diff --git a/lib/galaxy/dependencies/pinned-requirements.txt b/lib/galaxy/dependencies/pinned-requirements.txt
index 1aaed9f9450..1ac221dfb5b 100644
--- a/lib/galaxy/dependencies/pinned-requirements.txt
+++ b/lib/galaxy/dependencies/pinned-requirements.txt
@@ -1,5 +1,6 @@
 --extra-index-url https://wheels.galaxyproject.org/simple
 
+a2wsgi==1.4.0; python_version >= "3.6" and python_version < "4.0"
 adal==1.2.7
 aiofiles==0.8.0; python_version >= "3.6" and python_version < "4.0"
 amqp==5.0.9; python_version >= "3.7"
diff --git a/lib/galaxy/webapps/galaxy/fast_app.py b/lib/galaxy/webapps/galaxy/fast_app.py
index ebb28541572..ca99572d429 100644
--- a/lib/galaxy/webapps/galaxy/fast_app.py
+++ b/lib/galaxy/webapps/galaxy/fast_app.py
@@ -1,8 +1,8 @@
 from pathlib import Path
 from typing import cast
 
+from a2wsgi import WSGIMiddleware
 from fastapi import FastAPI, Request
-from fastapi.middleware.wsgi import WSGIMiddleware
 from starlette.middleware.cors import CORSMiddleware
 from starlette.responses import (
     FileResponse,
diff --git a/lib/galaxy/webapps/reports/fast_app.py b/lib/galaxy/webapps/reports/fast_app.py
index f5affed37a9..e03fb02fdd3 100644
--- a/lib/galaxy/webapps/reports/fast_app.py
+++ b/lib/galaxy/webapps/reports/fast_app.py
@@ -1,5 +1,5 @@
+from a2wsgi import WSGIMiddleware
 from fastapi import FastAPI
-from fastapi.middleware.wsgi import WSGIMiddleware
 
 from galaxy.webapps.base.api import (
     add_exception_handler,
diff --git a/lib/tool_shed/webapp/fast_app.py b/lib/tool_shed/webapp/fast_app.py
index 2d0c70d4d97..9fef316b764 100644
--- a/lib/tool_shed/webapp/fast_app.py
+++ b/lib/tool_shed/webapp/fast_app.py
@@ -1,5 +1,5 @@
+from a2wsgi import WSGIMiddleware
 from fastapi import FastAPI
-from fastapi.middleware.wsgi import WSGIMiddleware
 
 from galaxy.webapps.base.api import (
     add_exception_handler,
diff --git a/packages/webapps/requirements.txt b/packages/webapps/requirements.txt
index 7a195cd8db1..a6c4c554e94 100644
--- a/packages/webapps/requirements.txt
+++ b/packages/webapps/requirements.txt
@@ -1,3 +1,4 @@
+a2wsgi
 galaxy-app
 Cheetah3
 fastapi>=0.68.2,!=0.69.0,!=0.70.0,!=0.70.1
diff --git a/pyproject.toml b/pyproject.toml
index f0cc84f1e01..09114e68eec 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -14,6 +14,7 @@ name = "galaxyproject"
 url = "https://wheels.galaxyproject.org/simple"
 
 [tool.poetry.dependencies]
+a2wsgi = "*"
 aiofiles = "*"
 Babel = "*"
 bdbag = "*"
diff --git a/test/unit/webapps/test_send_file.py b/test/unit/webapps/test_send_file.py
index 2ee0bdd248c..755ce16a120 100644
--- a/test/unit/webapps/test_send_file.py
+++ b/test/unit/webapps/test_send_file.py
@@ -1,8 +1,8 @@
 import tempfile
 
 import pytest
+from a2wsgi import WSGIMiddleware
 from fastapi.applications import FastAPI
-from fastapi.middleware.wsgi import WSGIMiddleware
 from fastapi.testclient import TestClient
 
 from galaxy.util.bunch import Bunch