diff --git a/.isort.cfg b/.isort.cfg
new file mode 100644
index 00000000000..2197e0fc2f6
--- /dev/null
+++ b/.isort.cfg
@@ -0,0 +1,11 @@
+[settings]
+force_alphabetical_sort_within_sections=true
+multi_line_output=3
+force_grid_wrap=2
+include_trailing_comma=true
+src_paths=lib
+known_first_party=galaxy_test
+no_lines_before=LOCALFOLDER
+reverse_relative=true
+skip_gitignore=true
+line_length=200
diff --git a/pyproject.toml b/pyproject.toml
index f172449c4a4..0a19ddcc294 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -133,15 +133,3 @@ testfixtures = "*"
 tuspy = "*"
 twill = "*"
 watchdog = "*"
-
-[tool.isort]
-force_alphabetical_sort_within_sections = true
-multi_line_output = 3
-force_grid_wrap = 2
-include_trailing_comma = true
-src_paths = ['lib']
-known_first_party = ['galaxy_test']
-no_lines_before = ['LOCALFOLDER']
-reverse_relative = true
-skip_gitignore = true
-line_length = 200