diff --git a/lib/galaxy/datatypes/anvio.py b/lib/galaxy/datatypes/anvio.py
index 51c8f9eecd7..a4fb4267977 100644
--- a/lib/galaxy/datatypes/anvio.py
+++ b/lib/galaxy/datatypes/anvio.py
@@ -5,7 +5,6 @@ https://github.com/merenlab/anvio
 import glob
 import logging
 import os
-import sys
 from typing import Optional
 
 from galaxy.datatypes.metadata import MetadataElement
@@ -168,9 +167,3 @@ class AnvioSamplesDB(AnvioDB):
     _anvio_basename = "SAMPLES.db"
     MetadataElement(name="anvio_basename", default=_anvio_basename, desc="Basename", readonly=True)
     file_ext = "anvio_samples_db"
-
-
-if __name__ == "__main__":
-    import doctest
-
-    doctest.testmod(sys.modules[__name__])
diff --git a/lib/galaxy/datatypes/assembly.py b/lib/galaxy/datatypes/assembly.py
index c3855350ecf..83ce0befae2 100644
--- a/lib/galaxy/datatypes/assembly.py
+++ b/lib/galaxy/datatypes/assembly.py
@@ -7,7 +7,6 @@ for velvet assembler tool in galaxy
 import logging
 import os
 import re
-import sys
 
 from galaxy.datatypes import (
     data,
@@ -242,9 +241,3 @@ class Velvet(Html):
     def set_meta(self, dataset, **kwd):
         Html.set_meta(self, dataset, **kwd)
         self.regenerate_primary_file(dataset)
-
-
-if __name__ == "__main__":
-    import doctest
-
-    doctest.testmod(sys.modules[__name__])
diff --git a/lib/galaxy/datatypes/binary.py b/lib/galaxy/datatypes/binary.py
index ca65077dd31..10d99f71ef0 100644
--- a/lib/galaxy/datatypes/binary.py
+++ b/lib/galaxy/datatypes/binary.py
@@ -9,7 +9,6 @@ import os
 import shutil
 import struct
 import subprocess
-import sys
 import tarfile
 import tempfile
 import zipfile
@@ -4196,9 +4195,3 @@ class HexrdEtaOmeNpz(Npz):
             return dataset.peek
         except Exception:
             return "Binary Numpy npz file (%s)" % (nice_size(dataset.get_size()))
-
-
-if __name__ == "__main__":
-    import doctest
-
-    doctest.testmod(sys.modules[__name__])
diff --git a/lib/galaxy/datatypes/genetics.py b/lib/galaxy/datatypes/genetics.py
index 1c10d06ab77..17c11eef8a4 100644
--- a/lib/galaxy/datatypes/genetics.py
+++ b/lib/galaxy/datatypes/genetics.py
@@ -1236,9 +1236,3 @@ class AllegroLOD(LinkageStudies):
                 return False
 
         return True
-
-
-if __name__ == "__main__":
-    import doctest
-
-    doctest.testmod(sys.modules[__name__])
diff --git a/lib/galaxy/datatypes/interval.py b/lib/galaxy/datatypes/interval.py
index e0240ddf300..32585c8af5f 100644
--- a/lib/galaxy/datatypes/interval.py
+++ b/lib/galaxy/datatypes/interval.py
@@ -1727,9 +1727,3 @@ class ScIdx(Tabular):
         if count >= 1:
             return True
         return False
-
-
-if __name__ == "__main__":
-    import doctest
-
-    doctest.testmod(sys.modules[__name__])
diff --git a/lib/galaxy/datatypes/mothur.py b/lib/galaxy/datatypes/mothur.py
index f1ac6c353e6..14a0f0b8f0b 100644
--- a/lib/galaxy/datatypes/mothur.py
+++ b/lib/galaxy/datatypes/mothur.py
@@ -3,7 +3,6 @@ Mothur Metagenomics Datatypes
 """
 import logging
 import re
-import sys
 
 from galaxy.datatypes.data import Text
 from galaxy.datatypes.metadata import MetadataElement
@@ -1064,9 +1063,3 @@ class SffFlow(Tabular):
         except Exception as exc:
             out = f"Can't create peek: {unicodify(exc)}"
         return out
-
-
-if __name__ == "__main__":
-    import doctest
-
-    doctest.testmod(sys.modules[__name__])
diff --git a/lib/galaxy/datatypes/neo4j.py b/lib/galaxy/datatypes/neo4j.py
index 31a35930c3b..041021a2972 100644
--- a/lib/galaxy/datatypes/neo4j.py
+++ b/lib/galaxy/datatypes/neo4j.py
@@ -2,7 +2,6 @@
 Neo4j Composite Dataset
 """
 import logging
-import sys
 
 from galaxy.datatypes.data import Data
 from galaxy.datatypes.images import Html
@@ -132,9 +131,3 @@ class Neo4jDBzip(Neo4j, Data):
         self.add_composite_file(
             "%s.zip", description="neostore zip", substitute_name_with_metadata="reference_name", is_binary=True
         )
-
-
-if __name__ == "__main__":
-    import doctest
-
-    doctest.testmod(sys.modules[__name__])
diff --git a/lib/galaxy/datatypes/sniff.py b/lib/galaxy/datatypes/sniff.py
index fb7ebeb25cc..5839b5a2927 100644
--- a/lib/galaxy/datatypes/sniff.py
+++ b/lib/galaxy/datatypes/sniff.py
@@ -10,7 +10,6 @@ import os
 import re
 import shutil
 import struct
-import sys
 import tempfile
 import zipfile
 from functools import partial
@@ -942,9 +941,3 @@ DECOMPRESSION_FUNCTIONS: Dict[str, Decompress] = dict(gzip=gzip.GzipFile, bz2=bz
 
 class InappropriateDatasetContentError(Exception):
     pass
-
-
-if __name__ == "__main__":
-    import doctest
-
-    doctest.testmod(sys.modules[__name__])
diff --git a/lib/galaxy/tool_util/deps/mulled/get_tests.py b/lib/galaxy/tool_util/deps/mulled/get_tests.py
index 02df99661fe..895995264a7 100644
--- a/lib/galaxy/tool_util/deps/mulled/get_tests.py
+++ b/lib/galaxy/tool_util/deps/mulled/get_tests.py
@@ -5,7 +5,6 @@ searches for tests for packages in the bioconda-recipes repo and on Anaconda, lo
 
 A shallow search (default for singularity and conda generation scripts) just checks once on Anaconda for the specified version.
 """
-# import doctest
 import json
 import logging
 import tarfile
diff --git a/lib/galaxy/util/__init__.py b/lib/galaxy/util/__init__.py
index 895c5bd0769..9844fed3404 100644
--- a/lib/galaxy/util/__init__.py
+++ b/lib/galaxy/util/__init__.py
@@ -1852,9 +1852,3 @@ class StructuredExecutionTimer:
     @property
     def elapsed(self):
         return time.time() - self.begin
-
-
-if __name__ == "__main__":
-    import doctest
-
-    doctest.testmod(sys.modules[__name__], verbose=False)