diff --git a/lib/galaxy/dependencies/pipfiles/default/Pipfile b/lib/galaxy/dependencies/pipfiles/default/Pipfile
index bf8281d506c..0b669e32aaa 100644
--- a/lib/galaxy/dependencies/pipfiles/default/Pipfile
+++ b/lib/galaxy/dependencies/pipfiles/default/Pipfile
@@ -25,7 +25,7 @@ Sphinx = "*"
 sphinx_markdown_tables = "*"
 sphinx_rtd_theme = "*"
 testfixtures = "*"
-twill = {version = "==0.9.1", markers = "python_version < '3'"}
+twill = "*"
 watchdog = "*"
 python-irodsclient = "*"
 
diff --git a/lib/galaxy/dependencies/pipfiles/default/pinned-dev-requirements.txt b/lib/galaxy/dependencies/pipfiles/default/pinned-dev-requirements.txt
index 13e8e7864c7..a66925ec2f3 100644
--- a/lib/galaxy/dependencies/pipfiles/default/pinned-dev-requirements.txt
+++ b/lib/galaxy/dependencies/pipfiles/default/pinned-dev-requirements.txt
@@ -57,7 +57,7 @@ sphinx-rtd-theme==0.4.3
 sphinx==1.8.5
 sphinxcontrib-websupport==1.1.2
 testfixtures==6.14.0
-twill==0.9.1 ; python_version < '3'
+twill==2.0
 typing==3.7.4.1 ; python_version < '3.5'
 urllib3==1.25.8
 watchdog==0.10.2