diff --git a/lib/galaxy/dependencies/pipfiles/default/Pipfile b/lib/galaxy/dependencies/pipfiles/default/Pipfile
index 3c3ce61385a..67964f85612 100644
--- a/lib/galaxy/dependencies/pipfiles/default/Pipfile
+++ b/lib/galaxy/dependencies/pipfiles/default/Pipfile
@@ -13,7 +13,6 @@ gunicorn = "*"
 lxml = "!=4.2.2"
 mock = "*"
 NoseHTML = "*"
-pygithub3 = {version = "*", markers = "python_version < '3'"}
 pytest = "*"
 pytest-cov = "*"
 pytest-html = "*"
diff --git a/lib/galaxy/dependencies/pipfiles/default/pinned-dev-requirements.txt b/lib/galaxy/dependencies/pipfiles/default/pinned-dev-requirements.txt
index 7388ad1a691..cbf02baf09f 100644
--- a/lib/galaxy/dependencies/pipfiles/default/pinned-dev-requirements.txt
+++ b/lib/galaxy/dependencies/pipfiles/default/pinned-dev-requirements.txt
@@ -34,7 +34,6 @@ pluggy==0.13.1
 port-for==0.4
 psutil==5.6.7
 py==1.8.0
-pygithub3==0.5.1 ; python_version < '3'
 pygments==2.5.2
 pyparsing==2.4.5
 pytest-cov==2.8.1