diff --git a/lib/galaxy/config/sample/themes_conf.yml.sample b/lib/galaxy/config/sample/themes_conf.yml.sample
index 75b89ddf33e..4c219042177 100644
--- a/lib/galaxy/config/sample/themes_conf.yml.sample
+++ b/lib/galaxy/config/sample/themes_conf.yml.sample
@@ -13,11 +13,26 @@ blue:
       img: "/static/favicon.svg"
       img-secondary: null
 
+blue:
+  masthead:
+    color: "#2c3143"
+    text:
+      color: "#f8f9fa"
+      hover: gold
+      active: white
+    link:
+      color: transparent
+      hover: transparent
+      active: "#181a24"
+    logo:
+      img: "/static/favicon.svg"
+      img-secondary: null
+
 pride:
   masthead:
       color: >
         linear-gradient(120deg,
-          #2c3143 0px 130px,
+          #3c476d 0px 130px,
           #fdda0f 131px 139px,
           #fff 140px 148px,
           #f4b0c9 149px 157px,
@@ -26,8 +41,8 @@ pride:
           #000 176px 184px,
           transparent 185px),
         linear-gradient(270deg,
-          #2c3143 0px 110px,
-          #2c314300 110px),
+          #3c476d 0px 110px,
+          #3c476d00 110px),
         linear-gradient(180deg,
           #FE0000 16.66%,
           #FD8C00 16.66% 33.32%,
@@ -40,9 +55,9 @@ pride:
         hover: gold
         active: white
       link:
-        color: "#2c3143"
-        hover: "#25537b"
-        active: "#181a24"
+        color: "#3c476d"
+        hover: "#323a53"
+        active: "#6170a6"
       logo:
         img: "/static/favicon.svg"
         img-secondary: null