diff --git a/tools/samtools/sam_pileup.py b/tools/samtools/sam_pileup.py
index f84bd90d6e5..4e11f48d61d 100644
--- a/tools/samtools/sam_pileup.py
+++ b/tools/samtools/sam_pileup.py
@@ -4,15 +4,22 @@
Creates a pileup file from a bam file and a reference.
usage: %prog [options]
- -i, --input1=i: bam file
+ -p, --input1=p: bam file
-o, --output1=o: Output pileup
- -r, --ref=r: Reference file type
+ -R, --ref=R: Reference file type
-n, --ownFile=n: User-supplied fasta reference file
-d, --dbkey=d: dbkey of user-supplied file
-x, --indexDir=x: Index directory
-b, --bamIndex=b: BAM index file
+ -s, --lastCol=s: Print the mapping quality as the last column
+ -i, --indels=i: Only output lines containing indels
+ -M, --mapCap=M: Cap mapping quality
+ -c, --consensus=c: Call the consensus sequence using MAQ consensu model
+ -T, --theta=T: Theta paramter (error dependency coefficient)
+ -N, --hapNum=N: Number of haplotypes in sample
+ -r, --fraction=r: Expected fraction of differences between a pair of haplotypes
+ -I, --phredProb=I: Phred probability of an indel in sequencing/prep
-usage: %prog input1 output1 ref_type refFile ownFile dbkey index_dir bam_index
"""
import os, sys, tempfile
@@ -49,17 +56,20 @@ def __main__():
tmpf0bambai = '%s.bam.bai' % tmpf0.name
tmpf1 = tempfile.NamedTemporaryFile(dir=tmp_dir)
tmpf1fai = '%s.fai' % tmpf1.name
+ opts = '%s %s -M %s' % (('','-s')[options.lastCol=='yes'], ('','-i')[options.indels=='yes'], options.mapCap)
+ if options.consensus == 'yes':
+ opts += ' -c -T %s -N %s -r %s -I %s' % (options.theta, options.hapNum, options.fraction, options.phredProb)
cmd1 = None
cmd2 = 'cp %s %s; cp %s %s' % (options.input1, tmpf0bam, options.bamIndex, tmpf0bambai)
- cmd3 = 'samtools pileup -f %s %s > %s 2> /dev/null'
+ cmd3 = 'samtools pileup %s -f %s %s > %s 2> /dev/null'
if options.ref =='indexed':
full_path = "%s.fai" % seq_path
if not os.path.exists( full_path ):
stop_err( "No sequences are available for '%s', request them by reporting this error." % options.dbkey )
- cmd3 = cmd3 % (seq_path, tmpf0bam, options.output1)
+ cmd3 = cmd3 % (opts, seq_path, tmpf0bam, options.output1)
elif options.ref == 'history':
cmd1 = 'cp %s %s; cp %s.fai %s' % (options.ownFile, tmpf1.name, options.ownFile, tmpf1fai)
- cmd3 = cmd3 % (tmpf1.name, tmpf0bam, options.output1)
+ cmd3 = cmd3 % (opts, tmpf1.name, tmpf0bam, options.output1)
# index reference if necessary
if cmd1:
try:
diff --git a/tools/samtools/sam_pileup.xml b/tools/samtools/sam_pileup.xml
index 4054d1f1d56..90e0f5844ec 100644
--- a/tools/samtools/sam_pileup.xml
+++ b/tools/samtools/sam_pileup.xml
@@ -13,6 +13,21 @@
--dbkey=${input1.metadata.dbkey}
--indexDir=${GALAXY_DATA_INDEX_DIR}
--bamIndex=${input1.metadata.bam_index}
+ --lastCol=$lastCol
+ --indels=$indels
+ --mapCap=$mapCap
+ --consensus=$c.consensus
+ #if $c.consensus == "yes":
+ --theta=$c.theta
+ --hapNum=$c.hapNum
+ --fraction=$c.fraction
+ --phredProb=$c.phredProb
+ #else:
+ --theta="None"
+ --hapNum="None"
+ --fraction="None"
+ --phredProb="None"
+ #end if
@@ -31,6 +46,28 @@
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