diff --git a/.circleci/config.yml b/.circleci/config.yml
index acddc1aa54b..79e405da3e5 100644
--- a/.circleci/config.yml
+++ b/.circleci/config.yml
@@ -27,7 +27,7 @@ variables:
 jobs:
   get_code:
     docker:
-      - image: circleci/python:3.6
+      - image: circleci/python:3.7
     <<: *set_workdir
     steps:
       # Replace standard code checkout with shallow clone to speed things up.
@@ -85,7 +85,7 @@ jobs:
             - ~/repo
   validate_test_tools:
     docker:
-      - image: circleci/python:3.6
+      - image: circleci/python:3.7
     <<: *set_workdir
     steps:
       - *restore_repo_cache
@@ -95,7 +95,7 @@ jobs:
       - run: tox -e validate_test_tools
   test_galaxy_release:
     docker:
-      - image: circleci/python:3.6
+      - image: circleci/python:3.7
     <<: *set_workdir
     steps:
       - *restore_repo_cache
@@ -104,7 +104,7 @@ jobs:
       - run: tox -e test_galaxy_release
   test_galaxy_packages:
     docker:
-      - image: circleci/python:3.6
+      - image: circleci/python:3.7
     <<: *set_workdir
     steps:
       - *restore_repo_cache
diff --git a/.github/workflows/dependencies.yaml b/.github/workflows/dependencies.yaml
index 38399d42ca3..8cd1dd04051 100644
--- a/.github/workflows/dependencies.yaml
+++ b/.github/workflows/dependencies.yaml
@@ -9,7 +9,7 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        python-version: ['3.6']
+        python-version: ['3.7']
     steps:
       - uses: actions/checkout@v2
       - uses: actions/setup-python@v2
diff --git a/.github/workflows/first_startup.yaml b/.github/workflows/first_startup.yaml
index 4d04e43ca11..dd1fba26d77 100644
--- a/.github/workflows/first_startup.yaml
+++ b/.github/workflows/first_startup.yaml
@@ -19,10 +19,10 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.6', '3.9']
+        python-version: ['3.7', '3.10']
         webserver: ['uwsgi']
         include:
-          - python-version: '3.9'
+          - python-version: '3.10'
             webserver: 'dev'
     defaults:
       run:
diff --git a/.github/workflows/lint.yaml b/.github/workflows/lint.yaml
index 4c9d7cd1ad6..9dfd554cf3c 100644
--- a/.github/workflows/lint.yaml
+++ b/.github/workflows/lint.yaml
@@ -15,7 +15,7 @@ jobs:
     runs-on: ubuntu-latest
     strategy:
       matrix:
-        python-version: ['3.6', '3.9']
+        python-version: ['3.7', '3.10']
     steps:
       - uses: actions/checkout@v2
       - uses: actions/setup-python@v2
diff --git a/.github/workflows/osx_startup.yaml b/.github/workflows/osx_startup.yaml
index b7ffee4d64d..1100c557dbd 100644
--- a/.github/workflows/osx_startup.yaml
+++ b/.github/workflows/osx_startup.yaml
@@ -29,8 +29,8 @@ jobs:
       id: pip-cache
       with:
         path: ~/Library/Caches/pip
-        # scripts/common_startup.sh creates a conda env for Galaxy containing Python 3.6
-        key: pip-cache-3.6-${{ hashFiles('galaxy root/requirements.txt') }}
+        # scripts/common_startup.sh creates a conda env for Galaxy containing Python 3.7
+        key: pip-cache-3.7-${{ hashFiles('galaxy root/requirements.txt') }}
     - name: Cache tox env
       uses: actions/cache@v2
       with:
diff --git a/.github/workflows/unit.yaml b/.github/workflows/unit.yaml
index 7538f328f24..7a237ef3c91 100644
--- a/.github/workflows/unit.yaml
+++ b/.github/workflows/unit.yaml
@@ -18,7 +18,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        python-version: ['3.6', '3.9']
+        python-version: ['3.7', '3.10']
     steps:
       - uses: actions/checkout@v2
         with:
diff --git a/lib/galaxy/dependencies/dev-requirements.txt b/lib/galaxy/dependencies/dev-requirements.txt
index 75dcf95ee85..793e082e34a 100644
--- a/lib/galaxy/dependencies/dev-requirements.txt
+++ b/lib/galaxy/dependencies/dev-requirements.txt
@@ -3,57 +3,48 @@
 adal==1.2.7
 aiofiles==0.8.0; python_version >= "3.6" and python_version < "4.0"
 alabaster==0.7.12; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6"
-amqp==5.0.6; python_version >= "3.6"
-appdirs==1.4.4; python_version >= "3.6"
+amqp==5.0.6; python_version >= "3.7"
+anyio==3.4.0; python_full_version >= "3.6.2" and python_version >= "3.6"
+appdirs==1.4.4
 argcomplete==1.12.3; python_version >= "3.6" and python_version < "4"
 asgiref==3.4.1; python_version >= "3.6"
-async-exit-stack==1.0.1; python_version >= "3.6" and python_version < "3.7"
-async-generator==1.10; python_version < "3.7" and python_version >= "3.6"
-atomicwrites==1.4.0; python_version >= "3.6" and python_full_version < "3.0.0" and sys_platform == "win32" or sys_platform == "win32" and python_version >= "3.6" and python_full_version >= "3.4.0"
+async-generator==1.10; python_version >= "3.7" and python_version < "4.0"
+atomicwrites==1.4.0; python_version >= "3.7" and python_full_version < "3.0.0" and sys_platform == "win32" or sys_platform == "win32" and python_version >= "3.7" and python_full_version >= "3.4.0"
 attmap==0.13.2
-attrs==21.2.0; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version >= "3.6"
-autopage==0.4.0; python_version >= "3.6"
+attrs==21.2.0; python_version >= "3.7" and python_full_version < "3.0.0" and python_version < "4.0" or python_full_version >= "3.5.0" and python_version >= "3.7" and python_version < "4.0"
 babel==2.9.1; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.4.0")
 bagit-profile==1.3.1; python_version >= "2.7" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version < "4"
 bagit==1.8.1; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_full_version >= "3.5.0" and python_version < "4" and python_version >= "3.6"
 bcrypt==3.2.0; python_version >= "3.6"
 bdbag==1.6.3; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.5.0" and python_version < "4")
 beaker==1.11.0
-billiard==3.6.4.0; python_version >= "3.6"
+billiard==3.6.4.0; python_version >= "3.7"
 bioblend==0.16.0; python_version >= "3.6"
 bleach==4.1.0; python_version >= "3.6"
 boltons==21.0.0
-boto3==1.20.20; python_version >= "3.6"
 boto==2.49.0
-botocore==1.23.20; python_version >= "3.6"
 bx-python==0.8.12; python_version >= "3.6"
 cachecontrol==0.12.10; python_version >= "3.6" and python_version < "4"
-cached-property==1.5.2; python_version < "3.8" and python_version >= "3.6"
-cachetools==4.2.4; python_version >= "3.5" and python_version < "4.0" and (python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.6.0" and python_version >= "3.6")
-celery==5.1.2; python_version >= "3.6"
-certifi==2021.10.8; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_full_version >= "3.6.0" and python_version < "4" and python_version >= "3.6"
-cffi==1.15.0; implementation_name == "pypy" and python_version >= "3.6" and (python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6")
+cached-property==1.5.2; python_version < "3.8" and python_version >= "3.7"
+celery==5.2.1; python_version >= "3.7"
+certifi==2021.10.8; python_version >= "3.7" and python_full_version < "3.0.0" and python_version < "4" or python_full_version >= "3.6.0" and python_version < "4" and python_version >= "3.7"
+cffi==1.15.0
 charset-normalizer==2.0.9; python_full_version >= "3.6.0" and python_version >= "3.6" and python_version < "4"
 cheetah3==3.2.6.post1; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.4.0")
 circus==0.17.1
-click-didyoumean==0.0.3; python_version >= "3.6"
-click-plugins==1.1.1; python_version >= "3.6"
-click-repl==0.2.0; python_version >= "3.6"
-click==7.1.2; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version >= "3.6"
-cliff==3.10.0; python_version >= "3.6"
+click-didyoumean==0.3.0; python_full_version >= "3.6.2" and python_full_version < "4.0.0" and python_version >= "3.7"
+click-plugins==1.1.1; python_version >= "3.7"
+click-repl==0.2.0; python_version >= "3.7"
+click==8.0.3; python_full_version >= "3.6.2" and python_full_version < "4.0.0" and python_version >= "3.7"
 cloudauthz==0.6.0
-cloudbridge==2.2.0
-cmd2==2.3.3; python_version >= "3.6"
-colorama==0.4.4; python_version >= "3.6" and python_full_version < "3.0.0" and sys_platform == "win32" and python_version < "4.0" and platform_system == "Windows" and (python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6") or sys_platform == "win32" and python_version >= "3.6" and python_full_version >= "3.5.0" and python_version < "4.0" and platform_system == "Windows" and (python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6")
+cloudbridge==3.0.0
+colorama==0.4.4; sys_platform == "win32" and python_version >= "3.7" and python_full_version >= "3.6.2" and platform_system == "Windows" and python_full_version < "4.0.0" and (python_version >= "2.7" and python_full_version < "3.0.0" and platform_system == "Windows" or python_full_version >= "3.5.0" and platform_system == "Windows") and (python_version >= "3.7" and python_full_version < "3.0.0" and sys_platform == "win32" or sys_platform == "win32" and python_version >= "3.7" and python_full_version >= "3.5.0") and (python_version >= "3.6" and python_full_version < "3.0.0" and sys_platform == "win32" and (python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6") or sys_platform == "win32" and python_version >= "3.6" and (python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6") and python_full_version >= "3.5.0")
 coloredlogs==15.0.1; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_version >= "3.6" and python_version < "4" and python_full_version >= "3.5.0"
-commonmark==0.9.1; python_version >= "3.6" and python_version < "4.0"
-contextvars==2.4; python_version < "3.7" and python_version >= "3.6"
+commonmark==0.9.1; python_full_version >= "3.6.2" and python_full_version < "4.0.0"
 coverage==6.2; python_version >= "3.6"
-cryptography==36.0.0; python_version >= "3.6"
+cryptography==36.0.0; python_version >= "3.7" and python_full_version < "3.0.0" and python_version < "4" or python_full_version >= "3.6.0" and python_version < "4" and python_version >= "3.7"
 cwltest==2.2.20210901154959; python_version >= "3.6" and python_version < "4"
 cwltool==3.1.20211107152837; python_version >= "3.6" and python_version < "4"
-dataclasses==0.8; python_version >= "3.6" and python_version < "3.7" and python_full_version >= "3.6.1"
-debtcollector==2.3.0; python_version >= "3.6"
 decorator==5.1.0; python_version >= "3.6" and python_version < "4"
 defusedxml==0.7.1; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_full_version >= "3.5.0" and python_version >= "3.6" and python_version < "4"
 deprecated==1.2.13; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6"
@@ -61,7 +52,6 @@ deprecation==2.1.0
 dictobj==0.4
 docopt==0.6.2
 docutils==0.16; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.5.0")
-dogpile.cache==1.1.4; python_version >= "3.6"
 ecdsa==0.17.0; python_version >= "2.6" and python_full_version < "3.0.0" or python_full_version >= "3.3.0"
 edam-ontology==1.25.2
 fabric3==1.14.post1
@@ -72,37 +62,25 @@ fs==2.4.14
 funcsigs==1.0.2
 future==0.18.2; (python_version >= "2.6" and python_full_version < "3.0.0") or (python_full_version >= "3.3.0")
 galaxy-sequence-utils==1.1.5
-google-api-core==2.2.2; python_version >= "3.6"
-google-api-python-client==2.32.0; python_version >= "3.6"
-google-auth-httplib2==0.1.0; python_version >= "3.6"
-google-auth==2.3.3; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.6.0" and python_version >= "3.6"
-googleapis-common-protos==1.53.0; python_version >= "3.6"
 greenlet==1.1.2; python_version >= "3" and python_full_version < "3.0.0" and (platform_machine == "aarch64" or platform_machine == "ppc64le" or platform_machine == "x86_64" or platform_machine == "amd64" or platform_machine == "AMD64" or platform_machine == "win32" or platform_machine == "WIN32") and (python_version >= "2.7" and python_full_version < "3.0.0" or python_full_version >= "3.6.0") and (python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4.0" or python_version >= "3.6" and python_version < "4.0" and python_full_version >= "3.6.0") or python_version >= "3" and (platform_machine == "aarch64" or platform_machine == "ppc64le" or platform_machine == "x86_64" or platform_machine == "amd64" or platform_machine == "AMD64" or platform_machine == "win32" or platform_machine == "WIN32") and (python_version >= "2.7" and python_full_version < "3.0.0" or python_full_version >= "3.6.0") and (python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4.0" or python_version >= "3.6" and python_version < "4.0" and python_full_version >= "3.6.0") and python_full_version >= "3.5.0"
 gunicorn==20.1.0; python_version >= "3.5"
 gxformat2==0.15.0
-h11==0.12.0; python_version >= "3.6"
-h5py==3.1.0; python_version >= "3.6"
-httpcore==0.13.3; python_version >= "3.6"
-httplib2==0.20.2; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6"
-httpx==0.20.0; python_version >= "3.6"
+h11==0.12.0; python_version >= "3.7" and python_version < "4.0" and python_full_version >= "3.6.1"
+h5py==3.6.0; python_version >= "3.7"
+httpcore==0.14.3; python_version >= "3.6"
+httpx==0.21.1; python_version >= "3.6"
 humanfriendly==10.0; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_version >= "3.6" and python_version < "4" and python_full_version >= "3.5.0"
-idna==3.3; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_full_version >= "3.6.0" and python_version >= "3.6" and python_version < "4"
+idna==3.3; python_version >= "3.7" and python_version < "4.0" and python_full_version >= "3.6.2"
 imagesize==1.3.0; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6"
-immutables==0.16; python_version >= "3.6" and python_version < "3.7"
-importlib-metadata==4.8.2; python_version < "3.8" and python_version >= "3.6" and (python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "3.8" or python_full_version >= "3.6.0" and python_version < "3.8" and python_version >= "3.6") and (python_version == "3.6" or python_version == "3.7")
-importlib-resources==5.4.0; python_version < "3.7" and python_version >= "3.6"
-iniconfig==1.1.1; python_version >= "3.6"
+importlib-metadata==4.8.2; python_version == "3.7" and (python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "3.8" or python_full_version >= "3.6.0" and python_version < "3.8" and python_version >= "3.6")
+importlib-resources==5.4.0; python_version < "3.9" and python_version >= "3.7"
+iniconfig==1.1.1; python_version >= "3.7"
 isa-rwval==0.10.10
-iso8601==0.1.16; python_version >= "3.6"
-isodate==0.6.0; python_version >= "3.6" and python_version < "4"
+isodate==0.6.0; python_version >= "3.7" and python_version < "4"
 jinja2==3.0.3; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6"
-jmespath==0.10.0; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.3.0" and python_version >= "3.6"
-jsonpatch==1.32; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version >= "3.6"
-jsonpointer==2.2; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version >= "3.6"
-jsonschema==4.0.0
+jsonschema==4.2.1; python_version >= "3.7"
 junit-xml==1.9; python_version >= "3.6" and python_version < "4"
-keystoneauth1==4.4.0; python_version >= "3.6"
-kombu==5.1.0; python_version >= "3.6"
+kombu==5.2.2; python_version >= "3.7"
 lagom==1.7.0; python_version >= "3.6" and python_version < "4.0"
 lockfile==0.12.2; python_version >= "3.6" and python_version < "4"
 lxml==4.6.4; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.5.0")
@@ -111,65 +89,50 @@ markdown-it-reporter==0.0.2
 markdown==3.3.6; python_version >= "3.6"
 markupsafe==2.0.1; python_version >= "3.6"
 mercurial==6.0
-mirakuru==2.3.0; python_version >= "3.6"
+mirakuru==2.4.1; python_version >= "3.7"
 mistune==0.8.4; python_version >= "3.6" and python_version < "4"
 mrcfile==1.3.0
 msgpack==1.0.3; python_version >= "3.6" and python_version < "4"
-munch==2.5.0; python_version >= "3.6"
 mypy-extensions==0.4.3; python_version >= "3.6" and python_version < "4"
-netaddr==0.8.0; python_version >= "3.6"
-netifaces==0.11.0; python_version >= "3.6"
 networkx==2.5; python_version >= "3.6" and python_version < "4"
 nodeenv==1.6.0
 nose==1.3.7
 nosehtml==0.4.6
-numpy==1.19.5; python_version >= "3.6"
+numpy==1.21.1; python_version >= "3.7"
 oauthlib==3.1.1; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6"
-openstacksdk==0.61.0; python_version >= "3.6"
-os-client-config==2.1.0; python_version >= "3.6"
-os-service-types==1.7.0; python_version >= "3.6"
-osc-lib==2.4.2; python_version >= "3.6"
-oslo.config==8.7.1; python_version >= "3.6"
-oslo.context==3.4.0; python_version >= "3.6"
-oslo.i18n==5.1.0; python_version >= "3.6"
-oslo.log==4.6.1; python_version >= "3.6"
-oslo.serialization==4.2.0; python_version >= "3.6"
-oslo.utils==4.12.0; python_version >= "3.6"
+outcome==1.1.0; python_version >= "3.7" and python_version < "4.0"
 oyaml==1.0
-packaging==21.3; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6"
+packaging==21.3; python_version >= "3.7" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.7"
 paramiko==2.8.1
 parsley==1.3
 paste==3.5.0
 pastedeploy==2.1.1
-pbr==5.8.0; python_version >= "3.6"
-pluggy==1.0.0; python_version >= "3.6"
-port-for==0.5.0; python_version >= "3.6"
+pbr==5.8.0; python_version >= "2.6"
+pluggy==1.0.0; python_version >= "3.7"
+port-for==0.6.1; python_version >= "3.7"
 prettytable==2.4.0; python_version >= "3.6"
-prompt-toolkit==3.0.19; python_full_version >= "3.6.1" and python_version >= "3.6"
-protobuf==3.19.1; python_version >= "3.6"
+prompt-toolkit==3.0.23; python_full_version >= "3.6.2" and python_version >= "3.7"
 prov==1.5.1; python_version >= "3.6" and python_version < "4"
 psutil==5.8.0; (python_version >= "2.6" and python_full_version < "3.0.0") or (python_full_version >= "3.4.0")
 pulsar-galaxy-lib==0.14.13
-py==1.11.0; python_version >= "3.6" and python_full_version < "3.0.0" and implementation_name == "pypy" or python_full_version >= "3.5.0" and python_version >= "3.6" and implementation_name == "pypy"
-pyasn1-modules==0.2.8; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.6.0" and python_version >= "3.6"
-pyasn1==0.4.8; python_version >= "3.6" and python_version < "4" and (python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.6.0" and python_version >= "3.6")
-pycparser==2.21; python_version >= "3.6" and python_full_version < "3.0.0" and implementation_name == "pypy" and (python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6") or implementation_name == "pypy" and python_version >= "3.6" and (python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6") and python_full_version >= "3.4.0"
-pycryptodome==3.11.0; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.5.0")
+py==1.11.0; python_version >= "3.7" and python_full_version < "3.0.0" and implementation_name == "pypy" or python_full_version >= "3.5.0" and python_version >= "3.7" and implementation_name == "pypy"
+pyasn1==0.4.8; python_version >= "3.6" and python_version < "4"
+pycparser==2.21
+pycryptodome==3.12.0; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.5.0")
 pydantic==1.8.2; python_version >= "3.6" and python_version < "4.0" and python_full_version >= "3.6.1"
 pydot==1.4.2; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_version >= "3.6" and python_version < "4" and python_full_version >= "3.4.0"
 pyeventsystem==0.1.0
 pyfaidx==0.6.3.1
 pygithub==1.55; python_version >= "3.6"
-pygments==2.10.0; python_version >= "3.6" and python_version < "4.0" and (python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6")
-pyinotify==0.9.6; sys_platform != "win32" and sys_platform != "darwin" and sys_platform != "sunos5" and python_version >= "3.6"
+pygments==2.10.0; python_full_version >= "3.6.2" and python_full_version < "4.0.0" and python_version >= "3.6" and (python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6")
 pyjwt==2.3.0; python_version >= "3.6"
 pykwalify==1.8.0
 pynacl==1.4.0; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6"
-pyparsing==2.4.7; (python_version >= "2.6" and python_full_version < "3.0.0") or (python_full_version >= "3.3.0")
-pyperclip==1.8.2; python_version >= "3.6"
+pyopenssl==21.0.0; python_version >= "3.7" and python_full_version < "3.0.0" and python_version < "4" or python_full_version >= "3.6.0" and python_version < "4" and python_version >= "3.7"
+pyparsing==3.0.6; python_version >= "3.6"
 pyreadline3==3.3; sys_platform == "win32" and python_version >= "3.8" and (python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_version >= "3.6" and python_version < "4" and python_full_version >= "3.5.0")
-pyreadline==2.1; sys_platform == "win32" and python_version < "3.8" and (python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_version >= "3.6" and python_version < "4" and python_full_version >= "3.5.0") and python_version >= "3.6"
-pyrsistent==0.18.0; python_version >= "3.6"
+pyreadline==2.1; sys_platform == "win32" and python_version < "3.8" and (python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_version >= "3.6" and python_version < "4" and python_full_version >= "3.5.0")
+pyrsistent==0.18.0; python_version >= "3.7"
 pysam==0.18.0
 pytest-asyncio==0.16.0; python_version >= "3.6"
 pytest-celery==0.0.0
@@ -178,46 +141,39 @@ pytest-html==3.1.1; python_version >= "3.6"
 pytest-json-report==1.4.1
 pytest-metadata==1.11.0; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.6.0" and python_version >= "3.6"
 pytest-mock==3.6.1; python_version >= "3.6"
-pytest-postgresql==2.6.1; python_version >= "3.6"
+pytest-postgresql==4.0.0; python_version >= "3.7"
 pytest-pythonpath==0.7.3
 pytest-shard==0.1.2; python_version >= "3.6"
 pytest==6.2.5; python_version >= "3.6"
 python-dateutil==2.8.2; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_version >= "3.6" and python_version < "4" and python_full_version >= "3.3.0"
 python-irodsclient==1.0.0
 python-jose==3.3.0
-python-keystoneclient==4.3.0; python_version >= "3.6"
 python-multipart==0.0.5
-python-neutronclient==7.7.0; python_version >= "3.6"
-python-novaclient==17.6.0; python_version >= "3.6"
-python-swiftclient==3.13.0
 python3-openid==3.2.0; python_version >= "3.0"
-pytz==2021.3; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version < "4" and python_version >= "3.6"
+pytz==2021.3; python_version >= "3.7" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version < "4" and python_version >= "3.7"
 pyuwsgi==2.0.20
 pyyaml==6.0; python_version >= "3.6"
 pyzmq==22.3.0; python_version >= "3.6"
-rdflib==5.0.0; python_version >= "3.6" and python_version < "4"
+rdflib==6.0.2; python_version >= "3.7" and python_version < "4"
 recommonmark==0.7.1
 refgenconf==0.12.2
 repoze.lru==0.7
 requests-oauthlib==1.3.0; python_version >= "2.7" and python_full_version < "3.0.0" or python_full_version >= "3.4.0"
 requests-toolbelt==0.9.1; python_version >= "3.6"
 requests==2.26.0; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.6.0")
-requestsexceptions==1.4.0; python_version >= "3.6"
 responses==0.16.0; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.5.0")
 rfc3986==1.5.0; python_version >= "3.6"
-rich==10.11.0; python_version >= "3.6" and python_version < "4.0"
+rich==10.15.2; python_full_version >= "3.6.2" and python_full_version < "4.0.0"
 routes==2.5.1
-rsa==4.8; python_version >= "3.6" and python_version < "4" and (python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.6.0" and python_version >= "3.6")
+rsa==4.8; python_version >= "3.6" and python_version < "4"
 ruamel.yaml.clib==0.2.6; platform_python_implementation == "CPython" and python_version < "3.10" and python_version >= "3.6"
 ruamel.yaml==0.17.17; python_version >= "3.6" and python_version < "4"
-s3transfer==0.5.0; python_version >= "3.6"
 schema-salad==8.2.20211116214159; python_version >= "3.6" and python_version < "4"
-selenium==3.141.0
+selenium==4.1.0; python_version >= "3.7" and python_version < "4.0"
 setuptools-scm==5.0.2; python_version >= "2.7" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version < "4"
 shellescape==3.8.1; python_version >= "3.6" and python_version < "4"
-simplejson==3.17.6; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.3.0" and python_version >= "3.6"
-six==1.16.0; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_full_version >= "3.6.0" and python_version >= "3.6" and python_version < "4"
-sniffio==1.2.0; python_version >= "3.6"
+six==1.16.0; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.3.0")
+sniffio==1.2.0; python_version >= "3.7" and python_version < "4.0" and python_full_version >= "3.6.2"
 snowballstemmer==2.2.0; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6"
 social-auth-core==4.0.3
 sortedcontainers==2.4.0
@@ -237,34 +193,35 @@ sqlparse==0.4.2; python_version >= "3.5"
 starlette-context==0.3.3; python_version >= "3.7"
 starlette==0.14.2; python_version >= "3.6"
 statsd==3.3.0
-stevedore==3.5.0; python_version >= "3.6"
 svgwrite==1.4.1; python_version >= "3.6"
 tempita==0.5.2
 tenacity==8.0.1; python_version >= "3.6"
 testfixtures==6.18.3
-tifffile==2020.9.3; python_version >= "3.6"
-tinydb==4.5.2; python_version >= "3.5" and python_version < "4.0"
-toml==0.10.2; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.3.0" and python_version >= "3.6"
+tifffile==2021.11.2; python_version >= "3.7"
+tinydb==4.5.0; python_version >= "3.5" and python_version < "4.0"
+toml==0.10.2; python_version >= "3.7" and python_full_version < "3.0.0" or python_full_version >= "3.3.0" and python_version >= "3.7"
 tomli==1.2.2; python_version >= "3.6"
 tornado==6.1; python_version >= "3.5"
 tqdm==4.62.3; python_version >= "2.7" and python_full_version < "3.0.0" or python_full_version >= "3.4.0"
+trio-websocket==0.9.2; python_version >= "3.7" and python_version < "4.0"
+trio==0.19.0; python_version >= "3.7" and python_version < "4.0"
 tuspy==0.2.5
 tuswsgi==0.5.4
-twill==3.0
-typing-extensions==3.10.0.2
+twill==3.0.1
+typing-extensions==4.0.1; python_version >= "3.6"
 tzlocal==2.1; python_version >= "2.7" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version < "4"
 ubiquerg==0.6.2
-uritemplate==4.1.1; python_version >= "3.6"
-urllib3==1.26.7; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_full_version >= "3.6.0" and python_version < "4" and python_version >= "3.6"
+urllib3==1.26.7; python_version >= "3.7" and python_full_version < "3.0.0" and python_version < "4" or python_full_version >= "3.6.0" and python_version < "4" and python_version >= "3.7"
 uvicorn==0.15.0
-vine==5.0.0; python_version >= "3.6"
+vine==5.0.0; python_version >= "3.7"
 watchdog==2.1.6; python_version >= "3.6"
-wcwidth==0.2.5; python_full_version >= "3.6.1" and python_version >= "3.6"
+wcwidth==0.2.5; python_full_version >= "3.6.2" and python_version >= "3.7"
 webencodings==0.5.1; python_version >= "3.6"
 webob==1.8.7; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.3.0")
 whoosh==2.7.4
 wrapt==1.13.3; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version >= "3.6"
+wsproto==1.0.0; python_version >= "3.7" and python_version < "4.0" and python_full_version >= "3.6.1"
 xmlrunner==1.7.7
 yacman==0.8.4
-zipp==3.6.0; python_version < "3.7" and python_version >= "3.6" and (python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "3.8" or python_full_version >= "3.6.0" and python_version < "3.8" and python_version >= "3.6") and (python_version == "3.6" or python_version == "3.7")
+zipp==3.6.0; python_version == "3.7" and (python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "3.8" or python_full_version >= "3.6.0" and python_version < "3.8" and python_version >= "3.6")
 zipstream-new==1.1.8
diff --git a/lib/galaxy/dependencies/pinned-lint-requirements.txt b/lib/galaxy/dependencies/pinned-lint-requirements.txt
index 4c71f59b286..aa2c3c99a6c 100644
--- a/lib/galaxy/dependencies/pinned-lint-requirements.txt
+++ b/lib/galaxy/dependencies/pinned-lint-requirements.txt
@@ -19,7 +19,7 @@ types-docutils==0.17.1
 types-enum34==1.1.1
 types-ipaddress==1.0.1
 types-Markdown==3.3.9
-types-paramiko==2.8.2
+types-paramiko==2.8.3
 types-pkg-resources==0.1.3
 types-python-dateutil==2.8.3
 types-PyYAML==6.0.1
diff --git a/lib/galaxy/dependencies/pinned-requirements.txt b/lib/galaxy/dependencies/pinned-requirements.txt
index 206ae4c641d..ed6524a8bb7 100644
--- a/lib/galaxy/dependencies/pinned-requirements.txt
+++ b/lib/galaxy/dependencies/pinned-requirements.txt
@@ -2,61 +2,49 @@
 
 adal==1.2.7
 aiofiles==0.8.0; python_version >= "3.6" and python_version < "4.0"
-amqp==5.0.6; python_version >= "3.6"
-appdirs==1.4.4; python_version >= "3.6"
+amqp==5.0.6; python_version >= "3.7"
+appdirs==1.4.4
 argcomplete==1.12.3; python_version >= "3.6" and python_version < "4"
 asgiref==3.4.1; python_version >= "3.6"
-async-exit-stack==1.0.1; python_version >= "3.6" and python_version < "3.7"
-async-generator==1.10; python_version >= "3.6" and python_version < "3.7"
 attmap==0.13.2
-attrs==21.2.0; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version >= "3.6"
-autopage==0.4.0; python_version >= "3.6"
+attrs==21.2.0; python_version >= "3.7" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version >= "3.7"
 babel==2.9.1; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.4.0")
 bagit-profile==1.3.1; python_version >= "2.7" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version < "4"
 bagit==1.8.1; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_full_version >= "3.5.0" and python_version < "4" and python_version >= "3.6"
 bcrypt==3.2.0; python_version >= "3.6"
 bdbag==1.6.3; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.5.0" and python_version < "4")
 beaker==1.11.0
-billiard==3.6.4.0; python_version >= "3.6"
+billiard==3.6.4.0; python_version >= "3.7"
 bioblend==0.16.0; python_version >= "3.6"
 bleach==4.1.0; python_version >= "3.6"
 boltons==21.0.0
-boto3==1.20.20; python_version >= "3.6"
 boto==2.49.0
-botocore==1.23.20; python_version >= "3.6"
 bx-python==0.8.12; python_version >= "3.6"
 cachecontrol==0.12.10; python_version >= "3.6" and python_version < "4"
-cached-property==1.5.2; python_version < "3.8" and python_version >= "3.6"
-cachetools==4.2.4; python_version >= "3.5" and python_version < "4.0" and (python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.6.0" and python_version >= "3.6")
-celery==5.1.2; python_version >= "3.6"
+cached-property==1.5.2; python_version < "3.8" and python_version >= "3.7"
+celery==5.2.1; python_version >= "3.7"
 certifi==2021.10.8; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_full_version >= "3.6.0" and python_version < "4" and python_version >= "3.6"
 cffi==1.15.0; implementation_name == "pypy" and python_version >= "3.6" and (python_version >= "2.7" and python_full_version < "3.0.0" or python_full_version >= "3.4.0")
 charset-normalizer==2.0.9; python_full_version >= "3.6.0" and python_version >= "3.6" and python_version < "4"
 cheetah3==3.2.6.post1; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.4.0")
 circus==0.17.1
-click-didyoumean==0.0.3; python_version >= "3.6"
-click-plugins==1.1.1; python_version >= "3.6"
-click-repl==0.2.0; python_version >= "3.6"
-click==7.1.2; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version >= "3.6"
-cliff==3.10.0; python_version >= "3.6"
+click-didyoumean==0.3.0; python_full_version >= "3.6.2" and python_full_version < "4.0.0" and python_version >= "3.7"
+click-plugins==1.1.1; python_version >= "3.7"
+click-repl==0.2.0; python_version >= "3.7"
+click==8.0.3; python_full_version >= "3.6.2" and python_full_version < "4.0.0" and python_version >= "3.7"
 cloudauthz==0.6.0
-cloudbridge==2.2.0
-cmd2==2.3.3; python_version >= "3.6"
-colorama==0.4.4; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4.0" and platform_system == "Windows" or python_version >= "3.6" and python_version < "4.0" and python_full_version >= "3.5.0" and platform_system == "Windows"
+cloudbridge==3.0.0
+colorama==0.4.4; platform_system == "Windows" and python_version >= "3.7" and python_full_version >= "3.6.2" and python_full_version < "4.0.0" and (python_version >= "2.7" and python_full_version < "3.0.0" and platform_system == "Windows" or python_full_version >= "3.5.0" and platform_system == "Windows")
 coloredlogs==15.0.1; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_version >= "3.6" and python_version < "4" and python_full_version >= "3.5.0"
-commonmark==0.9.1; python_version >= "3.6" and python_version < "4.0"
-contextvars==2.4; python_version >= "3.6" and python_version < "3.7"
-cryptography==36.0.0; python_version >= "3.6"
+commonmark==0.9.1; python_full_version >= "3.6.2" and python_full_version < "4.0.0"
+cryptography==36.0.0; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_full_version >= "3.6.0" and python_version < "4" and python_version >= "3.6"
 cwltool==3.1.20211107152837; python_version >= "3.6" and python_version < "4"
-dataclasses==0.8; python_version >= "3.6" and python_version < "3.7" and python_full_version >= "3.6.1"
-debtcollector==2.3.0; python_version >= "3.6"
 decorator==5.1.0; python_version >= "3.6" and python_version < "4"
 defusedxml==0.7.1; python_version >= "3.0" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version >= "3.0"
 deprecation==2.1.0
 dictobj==0.4
 docopt==0.6.2
 docutils==0.16; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.5.0")
-dogpile.cache==1.1.4; python_version >= "3.6"
 ecdsa==0.17.0; python_version >= "2.6" and python_full_version < "3.0.0" or python_full_version >= "3.3.0"
 edam-ontology==1.25.2
 fabric3==1.14.post1
@@ -66,30 +54,18 @@ fs==2.4.14
 funcsigs==1.0.2
 future==0.18.2; (python_version >= "2.6" and python_full_version < "3.0.0") or (python_full_version >= "3.3.0")
 galaxy-sequence-utils==1.1.5
-google-api-core==2.2.2; python_version >= "3.6"
-google-api-python-client==2.32.0; python_version >= "3.6"
-google-auth-httplib2==0.1.0; python_version >= "3.6"
-google-auth==2.3.3; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.6.0" and python_version >= "3.6"
-googleapis-common-protos==1.53.0; python_version >= "3.6"
 greenlet==1.1.2; python_version >= "3" and python_full_version < "3.0.0" and (platform_machine == "aarch64" or platform_machine == "ppc64le" or platform_machine == "x86_64" or platform_machine == "amd64" or platform_machine == "AMD64" or platform_machine == "win32" or platform_machine == "WIN32") and (python_version >= "2.7" and python_full_version < "3.0.0" or python_full_version >= "3.6.0") and (python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4.0" or python_version >= "3.6" and python_version < "4.0" and python_full_version >= "3.6.0") or python_version >= "3" and (platform_machine == "aarch64" or platform_machine == "ppc64le" or platform_machine == "x86_64" or platform_machine == "amd64" or platform_machine == "AMD64" or platform_machine == "win32" or platform_machine == "WIN32") and (python_version >= "2.7" and python_full_version < "3.0.0" or python_full_version >= "3.6.0") and (python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4.0" or python_version >= "3.6" and python_version < "4.0" and python_full_version >= "3.6.0") and python_full_version >= "3.5.0"
 gxformat2==0.15.0
 h11==0.12.0; python_version >= "3.6"
-h5py==3.1.0; python_version >= "3.6"
-httplib2==0.20.2; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6"
+h5py==3.6.0; python_version >= "3.7"
 humanfriendly==10.0; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_version >= "3.6" and python_version < "4" and python_full_version >= "3.5.0"
 idna==3.3; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_full_version >= "3.6.0" and python_version >= "3.6" and python_version < "4"
-immutables==0.16; python_version >= "3.6" and python_version < "3.7"
-importlib-metadata==4.8.2; python_version < "3.8" and python_version >= "3.6" and (python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "3.8" or python_full_version >= "3.6.0" and python_version < "3.8" and python_version >= "3.6") and (python_version == "3.6" or python_version == "3.7")
-importlib-resources==5.4.0; python_version < "3.7" and python_version >= "3.6"
+importlib-metadata==4.8.2; python_version == "3.7" and (python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "3.8" or python_full_version >= "3.6.0" and python_version < "3.8" and python_version >= "3.6")
+importlib-resources==5.4.0; python_version < "3.9" and python_version >= "3.7"
 isa-rwval==0.10.10
-iso8601==0.1.16; python_version >= "3.6"
-isodate==0.6.0; python_version >= "3.6" and python_version < "4"
-jmespath==0.10.0; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.3.0" and python_version >= "3.6"
-jsonpatch==1.32; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version >= "3.6"
-jsonpointer==2.2; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version >= "3.6"
-jsonschema==4.0.0
-keystoneauth1==4.4.0; python_version >= "3.6"
-kombu==5.1.0; python_version >= "3.6"
+isodate==0.6.0; python_version >= "3.7" and python_version < "4"
+jsonschema==4.2.1; python_version >= "3.7"
+kombu==5.2.2; python_version >= "3.7"
 lagom==1.7.0; python_version >= "3.6" and python_version < "4.0"
 lockfile==0.12.2; python_version >= "3.6" and python_version < "4"
 lxml==4.6.4; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.5.0")
@@ -100,89 +76,63 @@ mercurial==6.0
 mistune==0.8.4; python_version >= "3.6" and python_version < "4"
 mrcfile==1.3.0
 msgpack==1.0.3; python_version >= "3.6" and python_version < "4"
-munch==2.5.0; python_version >= "3.6"
 mypy-extensions==0.4.3; python_version >= "3.6" and python_version < "4"
-netaddr==0.8.0; python_version >= "3.6"
-netifaces==0.11.0; python_version >= "3.6"
 networkx==2.5; python_version >= "3.6" and python_version < "4"
 nodeenv==1.6.0
 nose==1.3.7
-numpy==1.19.5; python_version >= "3.6"
+numpy==1.21.1; python_version >= "3.7"
 oauthlib==3.1.1; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.4.0" and python_version >= "3.6"
-openstacksdk==0.61.0; python_version >= "3.6"
-os-client-config==2.1.0; python_version >= "3.6"
-os-service-types==1.7.0; python_version >= "3.6"
-osc-lib==2.4.2; python_version >= "3.6"
-oslo.config==8.7.1; python_version >= "3.6"
-oslo.context==3.4.0; python_version >= "3.6"
-oslo.i18n==5.1.0; python_version >= "3.6"
-oslo.log==4.6.1; python_version >= "3.6"
-oslo.serialization==4.2.0; python_version >= "3.6"
-oslo.utils==4.12.0; python_version >= "3.6"
 oyaml==1.0
 packaging==21.3; python_version >= "3.6"
 paramiko==2.8.1
 parsley==1.3
 paste==3.5.0
 pastedeploy==2.1.1
-pbr==5.8.0; python_version >= "3.6"
-prettytable==2.4.0; python_version >= "3.6"
-prompt-toolkit==3.0.19; python_full_version >= "3.6.1" and python_version >= "3.6"
-protobuf==3.19.1; python_version >= "3.6"
+pbr==5.8.0; python_version >= "2.6"
+prompt-toolkit==3.0.23; python_full_version >= "3.6.2" and python_version >= "3.7"
 prov==1.5.1; python_version >= "3.6" and python_version < "4"
 psutil==5.8.0; (python_version >= "2.6" and python_full_version < "3.0.0") or (python_full_version >= "3.4.0")
 pulsar-galaxy-lib==0.14.13
 py==1.11.0; python_version >= "3.6" and python_full_version < "3.0.0" and implementation_name == "pypy" or implementation_name == "pypy" and python_version >= "3.6" and python_full_version >= "3.5.0"
-pyasn1-modules==0.2.8; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.6.0" and python_version >= "3.6"
-pyasn1==0.4.8; python_version >= "3.6" and python_version < "4" and (python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.6.0" and python_version >= "3.6")
+pyasn1==0.4.8; python_version >= "3.6" and python_version < "4"
 pycparser==2.21; python_version >= "3.6" and python_full_version < "3.0.0" and implementation_name == "pypy" and (python_version >= "2.7" and python_full_version < "3.0.0" or python_full_version >= "3.4.0") or implementation_name == "pypy" and python_version >= "3.6" and (python_version >= "2.7" and python_full_version < "3.0.0" or python_full_version >= "3.4.0") and python_full_version >= "3.4.0"
-pycryptodome==3.11.0; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.5.0")
+pycryptodome==3.12.0; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.5.0")
 pydantic==1.8.2; python_version >= "3.6" and python_version < "4.0" and python_full_version >= "3.6.1"
 pydot==1.4.2; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_version >= "3.6" and python_version < "4" and python_full_version >= "3.4.0"
 pyeventsystem==0.1.0
 pyfaidx==0.6.3.1
-pygments==2.10.0; python_version >= "3.6" and python_version < "4.0"
-pyinotify==0.9.6; sys_platform != "win32" and sys_platform != "darwin" and sys_platform != "sunos5" and python_version >= "3.6"
+pygments==2.10.0; python_full_version >= "3.6.2" and python_full_version < "4.0.0" and python_version >= "3.5"
 pyjwt==2.3.0; python_version >= "3.6"
 pykwalify==1.8.0
 pynacl==1.4.0; python_version >= "2.7" and python_full_version < "3.0.0" or python_full_version >= "3.4.0"
-pyparsing==2.4.7; (python_version >= "2.6" and python_full_version < "3.0.0") or (python_full_version >= "3.3.0")
-pyperclip==1.8.2; python_version >= "3.6"
+pyparsing==3.0.6; python_version >= "3.6"
 pyreadline3==3.3; sys_platform == "win32" and python_version >= "3.8" and (python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_version >= "3.6" and python_version < "4" and python_full_version >= "3.5.0")
-pyreadline==2.1; sys_platform == "win32" and python_version < "3.8" and (python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_version >= "3.6" and python_version < "4" and python_full_version >= "3.5.0") and python_version >= "3.6"
-pyrsistent==0.18.0; python_version >= "3.6"
+pyreadline==2.1; sys_platform == "win32" and python_version < "3.8" and (python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_version >= "3.6" and python_version < "4" and python_full_version >= "3.5.0")
+pyrsistent==0.18.0; python_version >= "3.7"
 pysam==0.18.0
 python-dateutil==2.8.2; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_version >= "3.6" and python_version < "4" and python_full_version >= "3.3.0"
 python-jose==3.3.0
-python-keystoneclient==4.3.0; python_version >= "3.6"
 python-multipart==0.0.5
-python-neutronclient==7.7.0; python_version >= "3.6"
-python-novaclient==17.6.0; python_version >= "3.6"
-python-swiftclient==3.13.0
 python3-openid==3.2.0; python_version >= "3.0"
-pytz==2021.3; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version < "4" and python_version >= "3.6"
+pytz==2021.3; python_version >= "3.7" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version < "4" and python_version >= "3.7"
 pyuwsgi==2.0.20
 pyyaml==6.0; python_version >= "3.6"
 pyzmq==22.3.0; python_version >= "3.6"
-rdflib==5.0.0; python_version >= "3.6" and python_version < "4"
+rdflib==6.0.2; python_version >= "3.7" and python_version < "4"
 refgenconf==0.12.2
 repoze.lru==0.7
 requests-oauthlib==1.3.0; python_version >= "2.7" and python_full_version < "3.0.0" or python_full_version >= "3.4.0"
 requests-toolbelt==0.9.1; python_version >= "3.6"
 requests==2.26.0; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.6.0")
-requestsexceptions==1.4.0; python_version >= "3.6"
-rfc3986==1.5.0; python_version >= "3.6"
-rich==10.11.0; python_version >= "3.6" and python_version < "4.0"
+rich==10.15.2; python_full_version >= "3.6.2" and python_full_version < "4.0.0"
 routes==2.5.1
-rsa==4.8; python_version >= "3.6" and python_version < "4" and (python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.6.0" and python_version >= "3.6")
+rsa==4.8; python_version >= "3.6" and python_version < "4"
 ruamel.yaml.clib==0.2.6; platform_python_implementation == "CPython" and python_version < "3.10" and python_version >= "3.6"
 ruamel.yaml==0.17.17; python_version >= "3.6" and python_version < "4"
-s3transfer==0.5.0; python_version >= "3.6"
 schema-salad==8.2.20211116214159; python_version >= "3.6" and python_version < "4"
 setuptools-scm==5.0.2; python_version >= "2.7" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version < "4"
 shellescape==3.8.1; python_version >= "3.6" and python_version < "4"
-simplejson==3.17.6; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.3.0" and python_version >= "3.6"
-six==1.16.0; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_full_version >= "3.6.0" and python_version >= "3.6" and python_version < "4"
+six==1.16.0; python_version >= "3.7" and python_full_version < "3.0.0" and python_version < "4" or python_full_version >= "3.4.0" and python_version >= "3.7" and python_version < "4"
 social-auth-core==4.0.3
 sortedcontainers==2.4.0
 sqlalchemy-migrate==0.13.0
@@ -191,26 +141,23 @@ sqlitedict==1.7.0
 sqlparse==0.4.2; python_version >= "3.5"
 starlette-context==0.3.3; python_version >= "3.7"
 starlette==0.14.2; python_version >= "3.6"
-stevedore==3.5.0; python_version >= "3.6"
 svgwrite==1.4.1; python_version >= "3.6"
 tempita==0.5.2
 tenacity==8.0.1; python_version >= "3.6"
-tifffile==2020.9.3; python_version >= "3.6"
+tifffile==2021.11.2; python_version >= "3.7"
 tornado==6.1; python_version >= "3.5"
 tqdm==4.62.3; python_version >= "2.7" and python_full_version < "3.0.0" or python_full_version >= "3.4.0"
 tuswsgi==0.5.4
-typing-extensions==3.10.0.2
+typing-extensions==4.0.1; python_version >= "3.6"
 tzlocal==2.1; python_version >= "2.7" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version < "4"
 ubiquerg==0.6.2
-uritemplate==4.1.1; python_version >= "3.6"
 urllib3==1.26.7; python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "4" or python_full_version >= "3.6.0" and python_version < "4" and python_version >= "3.6"
 uvicorn==0.15.0
-vine==5.0.0; python_version >= "3.6"
-wcwidth==0.2.5; python_full_version >= "3.6.1" and python_version >= "3.6"
+vine==5.0.0; python_version >= "3.7"
+wcwidth==0.2.5; python_full_version >= "3.6.2" and python_version >= "3.7"
 webencodings==0.5.1; python_version >= "3.6"
 webob==1.8.7; (python_version >= "2.7" and python_full_version < "3.0.0") or (python_full_version >= "3.3.0")
 whoosh==2.7.4
-wrapt==1.13.3; python_version >= "3.6" and python_full_version < "3.0.0" or python_full_version >= "3.5.0" and python_version >= "3.6"
 yacman==0.8.4
-zipp==3.6.0; python_version < "3.7" and python_version >= "3.6" and (python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "3.8" or python_full_version >= "3.6.0" and python_version < "3.8" and python_version >= "3.6") and (python_version == "3.6" or python_version == "3.7")
+zipp==3.6.0; python_version == "3.7" and (python_version >= "3.6" and python_full_version < "3.0.0" and python_version < "3.8" or python_full_version >= "3.6.0" and python_version < "3.8" and python_version >= "3.6")
 zipstream-new==1.1.8
diff --git a/lib/galaxy/dependencies/update_lint_requirements.sh b/lib/galaxy/dependencies/update_lint_requirements.sh
index 5c2e9b798e5..d477d941ab9 100755
--- a/lib/galaxy/dependencies/update_lint_requirements.sh
+++ b/lib/galaxy/dependencies/update_lint_requirements.sh
@@ -4,7 +4,7 @@ set -e
 THIS_DIRECTORY="$(cd "$(dirname "$0")" > /dev/null && pwd)"
 
 LINT_VENV=$(mktemp -d "${TMPDIR:-/tmp}/lint_venv.XXXXXXXXXX")
-python3 -m venv "${LINT_VENV}"
+python3.7 -m venv "${LINT_VENV}"
 . "${LINT_VENV}/bin/activate"
 pip install --upgrade pip setuptools
 pip install -r "${THIS_DIRECTORY}/lint-requirements.txt"
diff --git a/lib/galaxy_test/driver/driver_util.py b/lib/galaxy_test/driver/driver_util.py
index 461ecb39c1a..3c3ad913ae9 100644
--- a/lib/galaxy_test/driver/driver_util.py
+++ b/lib/galaxy_test/driver/driver_util.py
@@ -78,8 +78,7 @@ uwsgi:
 """)
 
 DEFAULT_LOCALES = "en"
-CAN_BUILD_ASGI_APP = sys.version_info[:2] >= (3, 7)
-USE_UVICORN = asbool(os.environ.get('GALAXY_TEST_USE_UVICORN', CAN_BUILD_ASGI_APP))
+USE_UVICORN = asbool(os.environ.get('GALAXY_TEST_USE_UVICORN', True))
 
 log = logging.getLogger("test_driver")
 
diff --git a/packages/app/setup.py b/packages/app/setup.py
index 78efdd6ebed..93f9af2344c 100644
--- a/packages/app/setup.py
+++ b/packages/app/setup.py
@@ -158,10 +158,10 @@ setup(
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 3",
-        'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
         'Programming Language :: Python :: 3.9',
+        'Programming Language :: Python :: 3.10',
     ],
     test_suite=TEST_DIR,
     tests_require=test_requirements
diff --git a/packages/auth/setup.py b/packages/auth/setup.py
index e9c127f99c4..2eafa480d0a 100644
--- a/packages/auth/setup.py
+++ b/packages/auth/setup.py
@@ -88,10 +88,10 @@ setup(
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 3",
-        'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
         'Programming Language :: Python :: 3.9',
+        'Programming Language :: Python :: 3.10',
     ],
     test_suite=TEST_DIR,
     tests_require=test_requirements
diff --git a/packages/containers/setup.py b/packages/containers/setup.py
index 754f18332f2..399aae00a47 100644
--- a/packages/containers/setup.py
+++ b/packages/containers/setup.py
@@ -89,10 +89,10 @@ setup(
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 3",
-        'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
         'Programming Language :: Python :: 3.9',
+        'Programming Language :: Python :: 3.10',
     ],
     test_suite=TEST_DIR,
     tests_require=test_requirements
diff --git a/packages/data/setup.py b/packages/data/setup.py
index 263571e25d5..6eb9d0f66c7 100644
--- a/packages/data/setup.py
+++ b/packages/data/setup.py
@@ -109,10 +109,10 @@ setup(
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 3",
-        'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
         'Programming Language :: Python :: 3.9',
+        'Programming Language :: Python :: 3.10',
     ],
     test_suite=TEST_DIR,
     tests_require=test_requirements
diff --git a/packages/files/setup.py b/packages/files/setup.py
index e3a0e90e275..bb48f5fec00 100644
--- a/packages/files/setup.py
+++ b/packages/files/setup.py
@@ -89,10 +89,10 @@ setup(
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 3",
-        'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
         'Programming Language :: Python :: 3.9',
+        'Programming Language :: Python :: 3.10',
     ],
     test_suite=TEST_DIR,
     tests_require=test_requirements
diff --git a/packages/job_execution/setup.py b/packages/job_execution/setup.py
index dbb6ec5e848..313826fa094 100644
--- a/packages/job_execution/setup.py
+++ b/packages/job_execution/setup.py
@@ -91,10 +91,10 @@ setup(
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 3",
-        'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
         'Programming Language :: Python :: 3.9',
+        'Programming Language :: Python :: 3.10',
     ],
     test_suite=TEST_DIR,
     tests_require=test_requirements
diff --git a/packages/job_metrics/setup.py b/packages/job_metrics/setup.py
index 34ed91f9981..6aaa5c24640 100644
--- a/packages/job_metrics/setup.py
+++ b/packages/job_metrics/setup.py
@@ -88,10 +88,10 @@ setup(
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 3",
-        'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
         'Programming Language :: Python :: 3.9',
+        'Programming Language :: Python :: 3.10',
     ],
     test_suite=TEST_DIR,
     tests_require=test_requirements
diff --git a/packages/meta/setup.py b/packages/meta/setup.py
index cdaa4c02446..69886c2feb5 100644
--- a/packages/meta/setup.py
+++ b/packages/meta/setup.py
@@ -116,10 +116,10 @@ setup(
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 3",
-        'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
         'Programming Language :: Python :: 3.9',
+        'Programming Language :: Python :: 3.10',
     ],
     test_suite=TEST_DIR,
     tests_require=test_requirements
diff --git a/packages/objectstore/setup.py b/packages/objectstore/setup.py
index 715191e6641..d7487978fbd 100644
--- a/packages/objectstore/setup.py
+++ b/packages/objectstore/setup.py
@@ -87,10 +87,10 @@ setup(
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 3",
-        'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
         'Programming Language :: Python :: 3.9',
+        'Programming Language :: Python :: 3.10',
     ],
     test_suite=TEST_DIR,
     tests_require=test_requirements
diff --git a/packages/selenium/setup.py b/packages/selenium/setup.py
index 01aac2114f5..e0ce6ffb455 100644
--- a/packages/selenium/setup.py
+++ b/packages/selenium/setup.py
@@ -90,10 +90,10 @@ setup(
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 3",
-        'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
         'Programming Language :: Python :: 3.9',
+        'Programming Language :: Python :: 3.10',
     ],
     test_suite=TEST_DIR,
     tests_require=test_requirements
diff --git a/packages/test_api/setup.py b/packages/test_api/setup.py
index a40273ce117..05e75f2eba3 100644
--- a/packages/test_api/setup.py
+++ b/packages/test_api/setup.py
@@ -87,10 +87,10 @@ setup(
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 3",
-        'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
         'Programming Language :: Python :: 3.9',
+        'Programming Language :: Python :: 3.10',
     ],
     test_suite=TEST_DIR,
     tests_require=test_requirements
diff --git a/packages/test_base/setup.py b/packages/test_base/setup.py
index cd5148d83a4..f1e501f2a0a 100644
--- a/packages/test_base/setup.py
+++ b/packages/test_base/setup.py
@@ -87,10 +87,10 @@ setup(
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 3",
-        'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
         'Programming Language :: Python :: 3.9',
+        'Programming Language :: Python :: 3.10',
     ],
     test_suite=TEST_DIR,
     tests_require=test_requirements
diff --git a/packages/test_driver/setup.py b/packages/test_driver/setup.py
index 0c3b9aec134..097c348490c 100644
--- a/packages/test_driver/setup.py
+++ b/packages/test_driver/setup.py
@@ -87,10 +87,10 @@ setup(
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 3",
-        'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
         'Programming Language :: Python :: 3.9',
+        'Programming Language :: Python :: 3.10',
     ],
     test_suite=TEST_DIR,
     tests_require=test_requirements
diff --git a/packages/test_selenium/setup.py b/packages/test_selenium/setup.py
index ff97e7dc707..ed5946425bc 100644
--- a/packages/test_selenium/setup.py
+++ b/packages/test_selenium/setup.py
@@ -88,10 +88,10 @@ setup(
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 3",
-        'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
         'Programming Language :: Python :: 3.9',
+        'Programming Language :: Python :: 3.10',
     ],
     test_suite=TEST_DIR,
     tests_require=test_requirements
diff --git a/packages/tool_util/setup.py b/packages/tool_util/setup.py
index c3eced55b8e..851770cb848 100644
--- a/packages/tool_util/setup.py
+++ b/packages/tool_util/setup.py
@@ -126,10 +126,10 @@ setup(
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 3",
-        'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
         'Programming Language :: Python :: 3.9',
+        'Programming Language :: Python :: 3.10',
     ],
     test_suite=TEST_DIR,
     tests_require=test_requirements
diff --git a/packages/util/setup.py b/packages/util/setup.py
index 40dc9978d31..e5cbb7ff406 100644
--- a/packages/util/setup.py
+++ b/packages/util/setup.py
@@ -96,10 +96,10 @@ setup(
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 3",
-        'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
         'Programming Language :: Python :: 3.9',
+        'Programming Language :: Python :: 3.10',
     ],
     test_suite=TEST_DIR,
     tests_require=test_requirements
diff --git a/packages/web_framework/setup.py b/packages/web_framework/setup.py
index 87c19e9d2e8..ebcecb09ff6 100644
--- a/packages/web_framework/setup.py
+++ b/packages/web_framework/setup.py
@@ -92,10 +92,10 @@ setup(
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 3",
-        'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
         'Programming Language :: Python :: 3.9',
+        'Programming Language :: Python :: 3.10',
     ],
     test_suite=TEST_DIR,
     tests_require=test_requirements
diff --git a/packages/web_stack/setup.py b/packages/web_stack/setup.py
index a69afcb4be1..e3020e83fb6 100644
--- a/packages/web_stack/setup.py
+++ b/packages/web_stack/setup.py
@@ -87,10 +87,10 @@ setup(
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 3",
-        'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
         'Programming Language :: Python :: 3.9',
+        'Programming Language :: Python :: 3.10',
     ],
     test_suite=TEST_DIR,
     tests_require=test_requirements
diff --git a/packages/webapps/setup.py b/packages/webapps/setup.py
index bc8ab520f0c..2cba3c31293 100644
--- a/packages/webapps/setup.py
+++ b/packages/webapps/setup.py
@@ -115,10 +115,10 @@ setup(
         'Topic :: Software Development :: Testing',
         'Natural Language :: English',
         "Programming Language :: Python :: 3",
-        'Programming Language :: Python :: 3.6',
         'Programming Language :: Python :: 3.7',
         'Programming Language :: Python :: 3.8',
         'Programming Language :: Python :: 3.9',
+        'Programming Language :: Python :: 3.10',
     ],
     test_suite=TEST_DIR,
     tests_require=test_requirements
diff --git a/pyproject.toml b/pyproject.toml
index 257ea66e861..137663cf33c 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -15,8 +15,6 @@ url = "https://wheels.galaxyproject.org/simple"
 
 [tool.poetry.dependencies]
 aiofiles = "*"
-async-generator = {version = "*", python = "~3.6"}
-async-exit-stack = {version = "*", python = "~3.6"}
 Babel = "*"
 bdbag = "*"
 Beaker = "1.11.0"
@@ -29,7 +27,6 @@ celery = "*"
 Cheetah3 = "!=3.2.6.post2"  # yanked release, https://github.com/python-poetry/poetry/issues/2453
 cloudauthz = "==0.6.0"
 cloudbridge = "*"
-contextvars = {version = "*", python = "~3.6"}
 circus = "*"
 cwltool = "==3.1.20211107152837"
 dictobj = "*"
@@ -42,7 +39,7 @@ fs = "*"
 future = "*"
 galaxy_sequence_utils = "*"
 gxformat2 = "*"
-h5py = "==3.1.0"  # Last version compatible with python 3.6. Also adding this constraint speeds up poetry resolution immensely
+h5py = "*"
 isa-rwval = "*"
 kombu = "*"
 lagom = "*"
@@ -63,7 +60,7 @@ pycryptodome = "*"
 pykwalify = "*"
 pyparsing = "*"
 pysam = "*"
-python = "^3.6.1"  # required by fastapi
+python = ">=3.7,<4"
 python-multipart = "*"  # required to support form parsing in FastAPI/Starlette
 pyuwsgi = "*"
 PyYAML = "*"
@@ -77,9 +74,9 @@ sqlalchemy-migrate = "*"
 sqlitedict = "*"
 sqlparse = "*"
 starlette = "*"
-starlette-context = {version = "*", python = ">=3.7"}
+starlette-context = "*"
 svgwrite = "*"
-tifffile = "<=2020.9.3"  # Last version compatible with python 3.6
+tifffile = "*"
 tuswsgi = "*"
 typing-extensions = "*"
 uvicorn = "*"
diff --git a/scripts/check_python.py b/scripts/check_python.py
index efb2d3076dc..085f9019122 100644
--- a/scripts/check_python.py
+++ b/scripts/check_python.py
@@ -7,14 +7,14 @@ import sys
 
 
 def check_python():
-    if sys.version_info[:2] >= (3, 6):
+    if sys.version_info[:2] >= (3, 7):
         # supported
         return
     else:
         version_string = '.'.join(str(_) for _ in sys.version_info[:3])
         msg = """\
 ERROR: Your Python version is: %s
-Galaxy is currently supported on Python >=3.6 .
+Galaxy is currently supported on Python >=3.7 .
 To run Galaxy, please install a supported Python version.
 If a supported version is already installed but is not your default,
 https://docs.galaxyproject.org/en/latest/admin/python.html contains instructions
diff --git a/scripts/common_startup.sh b/scripts/common_startup.sh
index 2e1f0da05ee..72c22ee5978 100755
--- a/scripts/common_startup.sh
+++ b/scripts/common_startup.sh
@@ -116,7 +116,7 @@ if [ $SET_VENV -eq 1 ] && [ $CREATE_VENV -eq 1 ]; then
                     echo "Creating Conda environment for Galaxy: $GALAXY_CONDA_ENV"
                     echo "To avoid this, use the --no-create-venv flag or set \$GALAXY_CONDA_ENV to an"
                     echo "existing environment before starting Galaxy."
-                    $CONDA_EXE create --yes --override-channels --channel conda-forge --channel defaults --name "$GALAXY_CONDA_ENV" 'python=3.6' 'pip>=19.0' 'virtualenv>=16'
+                    $CONDA_EXE create --yes --override-channels --channel conda-forge --channel defaults --name "$GALAXY_CONDA_ENV" 'python=3.7' 'pip>=19.0' 'virtualenv>=16'
                     unset __CONDA_INFO
                 fi
                 conda_activate
diff --git a/test/unit/webapps/test_request_scoped_sqlalchemy_sessions.py b/test/unit/webapps/test_request_scoped_sqlalchemy_sessions.py
index b1632d192fc..6c619059a74 100644
--- a/test/unit/webapps/test_request_scoped_sqlalchemy_sessions.py
+++ b/test/unit/webapps/test_request_scoped_sqlalchemy_sessions.py
@@ -1,7 +1,6 @@
 import asyncio
 import concurrent.futures
 import functools
-import sys
 import threading
 import time
 import uuid
@@ -149,10 +148,7 @@ async def test_request_scoped_sa_session_concurrent_requests_async():
 @pytest.mark.asyncio
 async def test_request_scoped_sa_session_concurrent_requests_and_background_thread():
     add_request_id_middleware(app)
-    if sys.version_info > (3, 6):
-        loop = asyncio.get_running_loop()
-    else:
-        loop = asyncio.get_event_loop()
+    loop = asyncio.get_running_loop()
     target = functools.partial(assert_scoped_session_is_thread_local, GX_APP)
     with concurrent.futures.ThreadPoolExecutor() as pool:
         background_pool = loop.run_in_executor(pool, target)
diff --git a/test/unit/webapps/test_webapp_base.py b/test/unit/webapps/test_webapp_base.py
index f799157e329..05f93634be9 100644
--- a/test/unit/webapps/test_webapp_base.py
+++ b/test/unit/webapps/test_webapp_base.py
@@ -3,7 +3,6 @@ Unit tests for ``galaxy.web.framework.webapp``
 """
 import logging
 import re
-import sys
 import unittest
 
 import galaxy.config
@@ -60,11 +59,7 @@ class GalaxyWebTransaction_Headers_TestCase(unittest.TestCase):
         hostnames = config._parse_allowed_origin_hostnames({
             "allowed_origin_hostnames": r"/host\d{2}/,geocities.com,miskatonic.edu"
         })
-        if sys.version_info >= (3, 7):
-            Pattern = re.Pattern
-        else:
-            Pattern = re._pattern_type
-        self.assertTrue(isinstance(hostnames[0], Pattern))
+        self.assertTrue(isinstance(hostnames[0], re.Pattern))
         self.assertTrue(isinstance(hostnames[1], str))
         self.assertTrue(isinstance(hostnames[2], str))