diff --git a/lib/galaxy/config/sample/tool_conf.xml.sample b/lib/galaxy/config/sample/tool_conf.xml.sample
index 3b1e24ff339..8e2f66de599 100644
--- a/lib/galaxy/config/sample/tool_conf.xml.sample
+++ b/lib/galaxy/config/sample/tool_conf.xml.sample
@@ -135,6 +135,7 @@
     <tool file="interactive/interactivetool_cellxgene.xml" />
     <tool file="interactive/interactivetool_ethercalc.xml" />
     <tool file="interactive/interactivetool_hicbrowser.xml" />
+    <tool file="interactive/interactivetool_jupytool.xml" />
     <tool file="interactive/interactivetool_jupyter_notebook.xml" />
     <tool file="interactive/interactivetool_neo4j.xml" />
     <tool file="interactive/interactivetool_phinch.xml" />