diff --git a/site/src/modules/resources/AgentRow.tsx b/site/src/modules/resources/AgentRow.tsx
index 6dd52bb31b..ae8dadcc39 100644
--- a/site/src/modules/resources/AgentRow.tsx
+++ b/site/src/modules/resources/AgentRow.tsx
@@ -41,6 +41,7 @@ import { TerminalLink } from "./TerminalLink/TerminalLink";
 import { useAgentContainers } from "./useAgentContainers";
 import { useAgentLogs } from "./useAgentLogs";
 import { VSCodeDesktopButton } from "./VSCodeDesktopButton/VSCodeDesktopButton";
+import { WildcardHostnameWarning } from "./WildcardHostnameWarning";
 
 interface AgentRowProps {
 	agent: WorkspaceAgent;
@@ -155,6 +156,10 @@ export const AgentRow: FC<AgentRowProps> = ({
 	// Check if any devcontainers have errors to gray out agent border
 	const hasDevcontainerErrors = devcontainers?.some((dc) => dc.error);
 
+	const hasSubdomainApps = agent.apps?.some((app) => app.subdomain);
+	const shouldShowWildcardWarning =
+		hasSubdomainApps && !proxy.proxy?.wildcard_hostname;
+
 	return (
 		<Stack
 			key={agent.id}
@@ -164,7 +169,8 @@ export const AgentRow: FC<AgentRowProps> = ({
 				styles.agentRow,
 				styles[`agentRow-${agent.status}`],
 				styles[`agentRow-lifecycle-${agent.lifecycle_state}`],
-				hasDevcontainerErrors && styles.agentRowWithErrors,
+				(hasDevcontainerErrors || shouldShowWildcardWarning) &&
+					styles.agentRowWithErrors,
 			]}
 		>
 			<header css={styles.header}>
@@ -226,6 +232,8 @@ export const AgentRow: FC<AgentRowProps> = ({
 					</section>
 				)}
 
+				{shouldShowWildcardWarning && <WildcardHostnameWarning />}
+
 				{shouldDisplayAppsSection && (
 					<section css={styles.apps}>
 						{shouldDisplayAgentApps && (
diff --git a/site/src/modules/resources/WildcardHostnameWarning.tsx b/site/src/modules/resources/WildcardHostnameWarning.tsx
new file mode 100644
index 0000000000..2210575ae4
--- /dev/null
+++ b/site/src/modules/resources/WildcardHostnameWarning.tsx
@@ -0,0 +1,84 @@
+import AlertTitle from "@mui/material/AlertTitle";
+import type { WorkspaceResource } from "api/typesGenerated";
+import { Alert, AlertDetail } from "components/Alert/Alert";
+import { Link } from "components/Link/Link";
+import { useProxy } from "contexts/ProxyContext";
+import { useAuthenticated } from "hooks/useAuthenticated";
+import type { FC } from "react";
+import { docs } from "utils/docs";
+
+interface WildcardHostnameWarningProps {
+	// If resources are provided, show template-focused warning
+	resources?: WorkspaceResource[];
+}
+
+export const WildcardHostnameWarning: FC<WildcardHostnameWarningProps> = ({
+	resources,
+}) => {
+	const { proxy } = useProxy();
+	const { permissions } = useAuthenticated();
+
+	const hasResources = Boolean(resources);
+	const canEditDeploymentConfig = Boolean(permissions.editDeploymentConfig);
+
+	if (proxy.proxy?.wildcard_hostname) {
+		return null;
+	}
+
+	if (hasResources) {
+		const hasSubdomainCoderApp = resources!.some((resource) => {
+			return resource.agents?.some((agent) =>
+				agent.apps?.some((app) => app.subdomain),
+			);
+		});
+
+		if (!hasSubdomainCoderApp) {
+			return null;
+		}
+	}
+
+	return (
+		<Alert
+			severity="warning"
+			className={
+				hasResources
+					? "rounded-none border-0 border-l-2 border-l-warning border-b-divider"
+					: undefined
+			}
+		>
+			<AlertTitle>Some workspace applications will not work</AlertTitle>
+			<AlertDetail>
+				<div>
+					{hasResources
+						? "This template contains coder_app resources with"
+						: "One or more apps in this workspace have"}{" "}
+					<code className="py-px px-1 bg-surface-tertiary rounded-sm text-content-primary">
+						subdomain = true
+					</code>
+					{canEditDeploymentConfig ? (
+						<>
+							, but subdomain applications are not configured. Users won't be
+							able to access these applications until you configure the{" "}
+							<code className="py-px px-1 bg-surface-tertiary rounded-sm text-content-primary">
+								--wildcard-access-url
+							</code>{" "}
+							flag when starting the Coder server.
+						</>
+					) : (
+						", which requires a Coder deployment with a Wildcard Access URL configured. Please contact your administrator."
+					)}
+				</div>
+				<div className="pt-2">
+					<Link
+						href={docs("/admin/networking/wildcard-access-url")}
+						target="_blank"
+					>
+						<span className="font-semibold">
+							Learn more about wildcard access URL
+						</span>
+					</Link>
+				</div>
+			</AlertDetail>
+		</Alert>
+	);
+};
diff --git a/site/src/pages/TemplateVersionEditorPage/TemplateVersionEditor.tsx b/site/src/pages/TemplateVersionEditorPage/TemplateVersionEditor.tsx
index 79c990544b..855ccbd19e 100644
--- a/site/src/pages/TemplateVersionEditorPage/TemplateVersionEditor.tsx
+++ b/site/src/pages/TemplateVersionEditorPage/TemplateVersionEditor.tsx
@@ -37,6 +37,7 @@ import {
 	ProvisionerAlert,
 } from "modules/provisioners/ProvisionerAlert";
 import { ProvisionerStatusAlert } from "modules/provisioners/ProvisionerStatusAlert";
+import { WildcardHostnameWarning } from "modules/resources/WildcardHostnameWarning";
 import { isBinaryData } from "modules/templates/TemplateFiles/isBinaryData";
 import { TemplateFileTree } from "modules/templates/TemplateFiles/TemplateFileTree";
 import { TemplateResourcesTable } from "modules/templates/TemplateResourcesTable/TemplateResourcesTable";
@@ -203,7 +204,7 @@ export const TemplateVersionEditor: FC<TemplateVersionEditorProps> = ({
 		if (logsContentRef.current) {
 			logsContentRef.current.scrollTop = logsContentRef.current.scrollHeight;
 		}
-	}, [buildLogs]);
+	}, [buildLogs, resources]);
 
 	useLeaveSiteWarning(dirty);
 
@@ -630,6 +631,10 @@ export const TemplateVersionEditor: FC<TemplateVersionEditorProps> = ({
 											logs={buildLogs}
 										/>
 									)}
+
+									{resources && (
+										<WildcardHostnameWarning resources={resources} />
+									)}
 								</div>
 							)}